Emergence of brown tides caused by Aureococcus anophagefferens Hargraves et Sieburth in China

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	Emergence of brown tides caused by Aureococcus anophagefferens Hargraves et Sieburth in China
	Zhang, Qing-Chun 1; Qiu, Li-Mei 2; Yu, Ren-Cheng 1; Kong, Fan-Zhou1 ; Wang, Yun-Feng 1; Yan, Tian 1; Gobler, Christopher J.3; Zhou, Ming-Jiang 1
刊名	HARMFUL ALGAE
	2012-09-01
卷号	19 页码:117-124
关键词	Aureococcus anophagefferens Brown tide Harmful algal bloom Mariculture 18S rDNA
ISSN号	1568-9883
通讯作者	Yu, RC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Large-scale blooms suspected to be "brown tides" occurred in early summer for three consecutive years from 2009 to 2011 in the coastal waters of Qinhuangdao, China, and had significant negative impacts on the shellfish mariculture industry. To identify the causative species of the blooms, phytoplankton samples were collected from regions with and without bloom in the coastal waters of Qinhuangdao in 2011, and clone libraries were built using eukaryote-specific 18S ribosomal RNA gene (18S rDNA). Altogether 50 clones, including 17 clones from bloom area and 33 clones from nearby regions without bloom were amplified. Blasted in GenBank, 17 clones amplified from the bloom area were assigned to Pelagophyceae (8 clones), Mediophyceae (2 clones), Cryptophyta (2 clones), Dinophyceae (2 clones) and unidentified eukaryotic species (3 clones). Those from the non-bloom site were assigned to Cryptophyta, Eustigmatophyceae, Prasinophyceae, Coscinodiscophyceae, Mediophyceae, Raphidophyceae and Dinophyceae, but not Pelagophyceae. All 8 pelagophyte clones from the bloom area were 99.7-100% similar to a single species, Aureococcus anophagefferens Hargraves et Sieburth, the causative species of brown tides on the east coast of USA. For nearly the entire length of the 18S rDNA, there were 0-6 base pair differences between the 8 amplicons and those of A. anophagefferens from USA. Furthermore, all of the 8 clones were clustered into the same well-supported clade with A. anophagefferens (posterior probability = 0.99) in a phylogenetic tree established for pelagophytes and other related microalgae. In our previous studies, the causative species of the bloom was tentatively identified as a pelagophyte, haptophyte or silicoflagellate, based on the pigment profile of the size-fractioned phytoplankton samples. Based on this study, we conclude that blooms in the coastal waters of Qinhuangdao of the Bohai Sea were brown tides caused by A. anophagefferens. As far as we know, this is the first report of brown tide events caused by A. anophagefferens in China, which is the third country in the world reporting A. anophagefferens blooms in addition to USA and South Africa. (c) 2012 Elsevier B.V. All rights reserved.
学科主题	Marine & Freshwater Biology
收录类别	SCI
原文出处	10.1016/j.hal.2012.06.007
语种	英语
WOS记录号	WOS:000309033700015
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12320]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.SUNY Stony Brook, Sch Marine & Atmospher Sci, New York, NY USA
推荐引用方式 GB/T 7714	Zhang, Qing-Chun,Qiu, Li-Mei,Yu, Ren-Cheng,et al. Emergence of brown tides caused by Aureococcus anophagefferens Hargraves et Sieburth in China[J]. HARMFUL ALGAE,2012,19:117-124.
APA	Zhang, Qing-Chun.,Qiu, Li-Mei.,Yu, Ren-Cheng.,Kong, Fan-Zhou.,Wang, Yun-Feng.,...&Zhou, Ming-Jiang.(2012).Emergence of brown tides caused by Aureococcus anophagefferens Hargraves et Sieburth in China.HARMFUL ALGAE,19,117-124.
MLA	Zhang, Qing-Chun,et al."Emergence of brown tides caused by Aureococcus anophagefferens Hargraves et Sieburth in China".HARMFUL ALGAE 19(2012):117-124.