Edwardsiella tarda DnaK: Expression, activity, and the basis for the construction of a bivalent live vaccine against E. tarda and Streptococcus iniae

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	Edwardsiella tarda DnaK: Expression, activity, and the basis for the construction of a bivalent live vaccine against E. tarda and Streptococcus iniae
	Hu, Yong-hua 1; Dang, Wei 1,2,3; Deng, Tian 1; Sun, Li 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-04-01
卷号	32 期号:4 页码:616-620
关键词	DnaK Edwardsiella tarda Streptococcus iniae Vaccine
ISSN号	1050-4648
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Edwardsiella tarda and Streptococcus iniae are important aquaculture pathogens that affect many species of farmed fish. In this study, we analyzed the expression, activity, and immunoprotective potential of E. tarda heat shock protein DnaK. We found that dnaK expression was upregulated under conditions of heat shock, oxidative stress, and infection of host cells. Recombinant DnaK (rDnaK) purified from Escherichia coli exhibited ATPase activity and induced protection in Japanese flounder (Paralichthys olivaceus) against lethal E. tarda challenge. On the basis of these results and our previous observation that a protective S. iniae antigen Sia10 which, when expressed heterogeneously in E. coli DH5 alpha, is secreted into the extracellular milieu, we constructed a chimeric antigen by fusing DnaK to Sia10. The resulting fusion protein Sia10-DnaK was expressed in DH5 alpha via the plasmid pTDK. Western blot analysis indicated that Sia10-DnaK was detected in the culture supernatant of DH5 alpha/pTDK. When flounder were vaccinated with live DH5 alpha/pTDK, strong protection was observed against both E. tarda and S. iniae. ELISA analysis detected specific serum antibody production in fish vaccinated with rDnaK and DH5 alpha/pTDK. Taken together, these results indicate that rDnaK is an intrinsic ATPase with immunoprotective property and that Sia10-DnaK delivered by a live bacterial host is an effective bivalent vaccine candidate against E. tarda and S. iniae infection. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2012.01.011
语种	英语
WOS记录号	WOS:000301827100014
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12314]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou Key Lab Anim Adaptat & Evolut, Hangzhou 310036, Zhejiang, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yong-hua,Dang, Wei,Deng, Tian,et al. Edwardsiella tarda DnaK: Expression, activity, and the basis for the construction of a bivalent live vaccine against E. tarda and Streptococcus iniae[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(4):616-620.
APA	Hu, Yong-hua,Dang, Wei,Deng, Tian,&Sun, Li.(2012).Edwardsiella tarda DnaK: Expression, activity, and the basis for the construction of a bivalent live vaccine against E. tarda and Streptococcus iniae.FISH & SHELLFISH IMMUNOLOGY,32(4),616-620.
MLA	Hu, Yong-hua,et al."Edwardsiella tarda DnaK: Expression, activity, and the basis for the construction of a bivalent live vaccine against E. tarda and Streptococcus iniae".FISH & SHELLFISH IMMUNOLOGY 32.4(2012):616-620.