Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions
	Zhang, Bao Yu 1; Jia, Zhao Jun 2; He, Lin Wen 1; Wang, Guang Ce 1; Peng, Guang 1
刊名	BOTANICA MARINA
	2012-08-01
卷号	55 期号:4 页码:427-434
关键词	desiccation mRNA expression level Porphyra yezoensis protein phosphatase 2C gene qPCR
ISSN号	0006-8055
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China.
英文摘要	To characterize the structure and function of the protein phosphatase 2C (PP2C) gene in Porphyra yezoensis, full-length cDNA of the locus (denoted pypp2c) was obtained by electronic cloning, rapid amplification of cDNA ends, and reverse-transcription PCR. The nucleotide sequence of PyPP2C was 1988 bp, including a 5'-untranslated region (UTR) of 301 bp, a 3'-UTR of 256 bp, and an open reading frame of 1431 bp that can be translated into 476 amino acids with a molecular mass of 50.96 kDa and a putative isoelectric point of 8.34. The amino acids had 30-36% identity with those encoded by PP2C genes in protozoa and higher plants. A PP2C-like catalytic domain was found in the 57-397 region, and several residues involved in the metal ion binding sites were conserved. mRNA expression levels in the three life stages of leafy thalli, filaments, and conchospores were examined by real-time fluorescent quantitative PCR. Highest expression of pypp2c occurred in conchospores. Gene expression levels were similar among the three life stages. pypp2c expression at different thallus relative water contents (RWCs) varied, with the highest expression occurring at 60% RWC.
学科主题	Plant Sciences ; Marine & Freshwater Biology
收录类别	SCI
原文出处	10.1515/bot-2011-0059
语种	英语
WOS记录号	WOS:000307499500009
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12299]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Bao Yu,Jia, Zhao Jun,He, Lin Wen,et al. Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions[J]. BOTANICA MARINA,2012,55(4):427-434.
APA	Zhang, Bao Yu,Jia, Zhao Jun,He, Lin Wen,Wang, Guang Ce,&Peng, Guang.(2012).Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions.BOTANICA MARINA,55(4),427-434.
MLA	Zhang, Bao Yu,et al."Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions".BOTANICA MARINA 55.4(2012):427-434.