A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential

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	A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential
	Hu, Yong-hua 1; Dang, Wei 1,2,3; Sun, Li 1
刊名	ARCHIVES OF MICROBIOLOGY
	2012-09-01
卷号	194 期号:9 页码:795-802
关键词	Pseudomonas fluorescens TonB-dependent receptor Virulence Vaccine
ISSN号	0302-8933
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Pseudomonas fluorescens is a Gram-negative bacterium and a common aquaculture pathogen. In this study, we identified from a pathogenic P. fluorescens strain a TonB-dependent outer membrane receptor, TdrA, as a secreted protein and examined its function and vaccine potential. TdrA is composed of 746 residues and possesses conserved structural domains of TonB-dependent outer membrane receptors. Quantitative real-time reverse transcriptase-PCR analysis showed that expression of tdrA was upregulated under conditions of iron starvation and during infection of host cells. Consistently, iron depletion induced increased production of TdrA protein in the outer membrane. Compared to the wild type, a tdrA-knock out mutant (1) was unable to grow in the absence of iron, (2) exhibited drastically attenuated overall bacterial virulence, and (3) was impaired in the ability to establish lethal infection in host tissues. Purified recombinant TdrA (rTdrA), when used as a subunit vaccine to immunize flounder, was able to induce strong protective immunity, including production of serum-specific antibodies that resulted in effective protection against lethal-dose P. fluorescens challenge. Together, these results indicate that TdrA is an outer membrane receptor and a protective immunogen that is likely to be involved in iron acquisition and, as a result, required for optimal bacterial virulence.
学科主题	Microbiology
收录类别	SCI
原文出处	10.1007/s00203-012-0812-3
语种	英语
WOS记录号	WOS:000307709100008
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12272]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou Key Lab Anim Adaptat & Evolut, Hangzhou 310036, Zhejiang, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yong-hua,Dang, Wei,Sun, Li. A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential[J]. ARCHIVES OF MICROBIOLOGY,2012,194(9):795-802.
APA	Hu, Yong-hua,Dang, Wei,&Sun, Li.(2012).A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential.ARCHIVES OF MICROBIOLOGY,194(9),795-802.
MLA	Hu, Yong-hua,et al."A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential".ARCHIVES OF MICROBIOLOGY 194.9(2012):795-802.