Comparative transcriptomic analysis of fireflies (Coleoptera: Lampyridae) to explore the molecular adaptations to fresh water | |
Zhang, Qi-Lin3; Li, Hong-Wei3; Dong, Zhi-Xiang3; Yang, Xiao-Jie3; Lin, Lian-Bing3; Chen, Jun-Yuan (陈均远)1; Yuan, Ming-Long2 | |
刊名 | MOLECULAR ECOLOGY |
2020-06-26 | |
页码 | 16 |
关键词 | aquatic firefly comparative transcriptomics freshwater environment metabolic efficiency molecular adaptation morphology |
ISSN号 | 0962-1083 |
DOI | 10.1111/mec.15504 |
英文摘要 | Aquatic insects are well adapted to freshwater environments, but the molecular basis of these adaptations remains largely unknown. Most firefly species (Coleoptera: Lampyridae) are terrestrial, but the larvae of several species are aquatic. Here, larval and adult transcriptomes fromAquatica leii(freshwater) andLychnuris praetexta(terrestrial) were generated to test whether the genes associated with metabolic efficiency and morphology have undergone adaptive evolution to fresh water. The aquatic fireflies had a significantly lower ratio of nonsynonymous to synonymous substitutions than the terrestrial insects, indicating a genomewide evolutionary constraint in the aquatic fireflies. We identified 341 fast-evolving genes and 116 positively selected genes in the aquatic fireflies. Of these, 76 genes exhibiting both fast evolution and positive selection were primarily involved in ATP production, energy metabolism and the hypoxia response. We identified 7,271 differentially expressed genes (DEGs) inA. leii(adults versus larvae) and 8,309 DEGs inL. praetexta(adults versus larvae). DEGs specific to the aquatic firefly (n = 1,445) were screened via interspecific comparisons (A. leiiversusL. praetexta) and were significantly enriched for genes involved in metabolic efficiency (e.g., ATP production, hypoxia, and immune responses) and certain aspects of morphology (e.g., cuticle chitin, tracheal and compound eye morphology). These results indicate that sequence and expression-level changes in genes associated with both metabolic efficiency and morphological attributes related to the freshwater lifestyle contributed to freshwater adaptation in fireflies. This study provides new insights into the molecular mechanisms of aquatic adaptation in insects. |
资助项目 | Natural Science Foundation of China[31960286] |
WOS关键词 | PHYLOGENETIC ANALYSIS ; ADAPTIVE EVOLUTION ; ANALYSIS REVEALS ; HYPOXIA ; ANNOTATION ; GYNAEPHORA ; EXPRESSION ; GENES ; RNA ; REGULATOR |
WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000543234300001 |
资助机构 | Natural Science Foundation of China |
内容类型 | 期刊论文 |
源URL | [http://ir.nigpas.ac.cn/handle/332004/32055] |
专题 | 中国科学院南京地质古生物研究所 |
通讯作者 | Zhang, Qi-Lin; Chen, Jun-Yuan (陈均远); Yuan, Ming-Long |
作者单位 | 1.Chinese Acad Sci, Nanjing Inst Geol & Paleontol, LPS, Nanjing, Peoples R China 2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou, Peoples R China 3.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming, Yunnan, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Qi-Lin,Li, Hong-Wei,Dong, Zhi-Xiang,et al. Comparative transcriptomic analysis of fireflies (Coleoptera: Lampyridae) to explore the molecular adaptations to fresh water[J]. MOLECULAR ECOLOGY,2020:16. |
APA | Zhang, Qi-Lin.,Li, Hong-Wei.,Dong, Zhi-Xiang.,Yang, Xiao-Jie.,Lin, Lian-Bing.,...&Yuan, Ming-Long.(2020).Comparative transcriptomic analysis of fireflies (Coleoptera: Lampyridae) to explore the molecular adaptations to fresh water.MOLECULAR ECOLOGY,16. |
MLA | Zhang, Qi-Lin,et al."Comparative transcriptomic analysis of fireflies (Coleoptera: Lampyridae) to explore the molecular adaptations to fresh water".MOLECULAR ECOLOGY (2020):16. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论