Neonatal cortical astrocytes possess intrinsic potential in neuronal conversion in defined media

doi:10.1038/s41401-020-00586-0

	Neonatal cortical astrocytes possess intrinsic potential in neuronal conversion in defined media
	Zeng, Peng 1; Hua, Qiu-hong 1; Gong, Jun-yuan 1; Shi, Chang-jie 1; Pi, Xiao-ping 2; Xie, Xin 2; Zhang, Ru 1
刊名	ACTA PHARMACOLOGICA SINICA
	2021-02-05
页码	12
关键词	cortical astrocytes neonatal neuronal conversion insulin heterogeneity
ISSN号	1671-4083
DOI	10.1038/s41401-020-00586-0
通讯作者	Xie, Xin(xiexin-2004@163.com) ; Zhang, Ru(ru.zhang@tongji.edu.cn)
英文摘要	Astrocytes are multifunctional brain cells responsible for maintaining the health and function of the central nervous system. Accumulating evidence suggests that astrocytes might be complementary source across different brain regions to supply new neurons during adult neurogenesis. In this study, we found that neonatal mouse cortical astrocytes can be directly converted into neurons when exposed to neurogenic differentiation culture conditions, with insulin being the most critical component. Detailed comparison studies between mouse cortical astrocytes and neuronal progenitor cells (NPCs) demonstrated the converted neuronal cells originate indeed from the astrocytes rather than NPCs. The neurons derived from mouse cortical astrocytes display typical neuronal morphologies, express neuronal markers and possess typical neuronal electrophysiological properties. More importantly, these neurons can survive and mature in the mouse brain in vivo. Finally, by comparing astrocytes from different brain regions, we found that only cortical astrocytes but not astrocytes from other brain regions such as hippocampus and cerebellum can be converted into neurons under the current condition. Altogether, our findings suggest that neonatal astrocytes from certain brain regions possess intrinsic potential to differentiate/transdifferentiate into neurons which may have clinical relevance in the future.
资助项目	Ministry of Science and Technology of China[2017YFA0104002] ; Chinese Academy of Sciences[XDA16010202] ; National Natural Science Foundation of China[81730099]
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000615156100002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/295695]
专题	中国科学院上海药物研究所
通讯作者	Xie, Xin; Zhang, Ru
作者单位	1.Tongji Univ, Shanghai Key Lab Signaling & Dis Res, Collaborat Innovat Ctr Brain Sci, Lab Receptor Based Biomed,Sch Life Sci & Technol, Shanghai 200092, Peoples R China 2.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Peng,Hua, Qiu-hong,Gong, Jun-yuan,et al. Neonatal cortical astrocytes possess intrinsic potential in neuronal conversion in defined media[J]. ACTA PHARMACOLOGICA SINICA,2021:12.
APA	Zeng, Peng.,Hua, Qiu-hong.,Gong, Jun-yuan.,Shi, Chang-jie.,Pi, Xiao-ping.,...&Zhang, Ru.(2021).Neonatal cortical astrocytes possess intrinsic potential in neuronal conversion in defined media.ACTA PHARMACOLOGICA SINICA,12.
MLA	Zeng, Peng,et al."Neonatal cortical astrocytes possess intrinsic potential in neuronal conversion in defined media".ACTA PHARMACOLOGICA SINICA (2021):12.