Intrinsic stability study of L-alpha-glycerylphosphorylcholine with HPLC method development and validation

doi:10.1016/j.jpba.2020.113468

	Intrinsic stability study of L-alpha-glycerylphosphorylcholine with HPLC method development and validation
	Zhao, Hongwei 1,2; Sun, Ling 1; Chen, Huanhuan 1,2; Xiang, Lili 1; Chen, Dongying 1,2
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2020-09-05
卷号	188 页码:6
关键词	L-alpha-Glycerylphosphorylcholine Phosphate ester bond Forced degradation HPLC Intrinsic stability Kinetics study
ISSN号	0731-7085
DOI	10.1016/j.jpba.2020.113468
通讯作者	Chen, Dongying(dychen@simm.ac.cn)
英文摘要	L-alpha-Glycerylphosphorylcholine (L-alpha-GPC) is effective to control the symptoms of cognitive decline for the patients of Alzheimer's disease. In this study, an HPLC method coupled with a refractive index detector was developed to evaluate the intrinsic stability of L-alpha-GPC. The separation of L-alpha-GPC and its major potential degradation products was achieved on a normal-phase silica gel column (4.6 mm x 250 mm) with the mobile phase consisting of methanol-20 mM ammonium formate aqueous solution (pH 3.2) (65:35, v/v) in isocratic mode. The HPLC method was validated satisfactorily with respect to precision, accuracy and robustness. It is found that L-alpha-GPC is stable under the photolytic, thermal, oxidative and acidic conditions, while relatively sensitive to alkaline condition due to the specific breakage of phosphate ester bond in the moiety of L-alpha-GPC. A preliminary kinetics study for the alkaline degradation was conducted with the corresponding kinetics parameters obtained. It can be concluded that the developed HPLC method is capable of distinguishing the stability difference between the two phosphate ester bonds characterized on the L-alpha-GPC chemical structure. (C) 2020 Elsevier B.V. All rights reserved.
WOS关键词	ALZHEIMERS-DISEASE ; DRUG ; PARAMETERS ; PRECURSOR
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000556785200048
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/292358]
专题	中国科学院上海药物研究所
通讯作者	Chen, Dongying
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Pharmaceut Anal, 555 Rd Zuchongzhi,Zhangjiang Hitech Pk, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Hongwei,Sun, Ling,Chen, Huanhuan,et al. Intrinsic stability study of L-alpha-glycerylphosphorylcholine with HPLC method development and validation[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2020,188:6.
APA	Zhao, Hongwei,Sun, Ling,Chen, Huanhuan,Xiang, Lili,&Chen, Dongying.(2020).Intrinsic stability study of L-alpha-glycerylphosphorylcholine with HPLC method development and validation.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,188,6.
MLA	Zhao, Hongwei,et al."Intrinsic stability study of L-alpha-glycerylphosphorylcholine with HPLC method development and validation".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 188(2020):6.