Exploration of the hepatoprotective chemical base of an orally administered herbal formulation (YCHT) in normal and CCl4-intoxicated liver injury rats. Part 2: Hepatic disposition in vivo and hepatoprotective activity in vitro | |
Tian, Xiaoting1,2; Liu, Huan1,2; Qiao, Shida1,2; Yin, Hao1,2; Chen, Mingcang1,2; Hu, Pei1,2; Wang, Yangyang1,2; Peng, Huige1,2; Liu, Fang1,2; Pan, Guoyu1,2 | |
刊名 | JOURNAL OF ETHNOPHARMACOLOGY |
2019-05-23 | |
卷号 | 236页码:161-172 |
关键词 | Yin-Chen-Hao Tang Hepatic disposition Pharmacokinetics Hepatoprotection HepG2 Carbon tetrachloride |
ISSN号 | 0378-8741 |
DOI | 10.1016/j.jep.2019.02.022 |
通讯作者 | Pan, Guoyu(gypan@simm.ac.cn) ; Huang, Chenggang(cghsimm@126.com) |
英文摘要 | Ethnopharmacological relevance: Yin-Chen-Hao Tang (YCHT) has been a very popular, hepatoprotective three-herb formula with an unclear chemical base. Aim of this study: To reveal the hepatoprotective chemical base of oral-dosed YCHT, we bridged the hepatic disposition of six compounds in vivo and their hepatoprotection in vitro. Materials and methods: In vivo, following the oral administration of YCHT in normal and CCl4-induced liver injury rats, the determinations of chlorogenic acid, 4-hydroxyacetophenone, geniposide, genipin, rhein and emodin were conducted in the portal vein plasma, the liver, and the systemic plasma. In vitro, the hepatoprotective activities of these compounds were determined in the CCl4-induced HepG2 cells. Results: Consistent with the highest content in YCHT, geniposide had the highest exposure in vivo. Inconsistent with the negligible content, rhein, 4-hydroxyacetophenone, emodin and genipin showed substantial hepatic accumulations. In contrast, chlorogenic acid, an ingredient that has a high content in YCHT, elicited no hepatic exposure. In normal rats, the hepatic disposition prevented the compounds entering into the systemic plasma from the portal vein plasma by 44.9-100%, except for rhein. CCl4-induced liver injury caused a decreased hepatic exposure of 4-hydroxyacetophenone, rhein and emodin by 50%. In vitro, all six compounds exerted the hepatoprotection by increasing cell viability, decreasing hepatic marker enzymes and inhibiting lipid peroxidation at varying levels. Conclusion Geniposide, rhein, emodin, 4-hydroxyacetophenone and genipin directly resisted liver injury in oral-dosed YCHT, while chlorogenic acid likely played an indirect role. This study proved that YCHT exerted hepatoprotection through multiple components and multiple actions. However, close attention should be paid to the possible side effects and oral dosage of YCHT in clinics. |
资助项目 | National Science and Technology Major Project of the Ministry of Science and Technology of China[2018ZX09731016-003] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2018ZX09201001-001-008] ; Shanghai Science and Technology Development Fund[18401931300] ; National Natural Science Foundation for Young Scientists of China[81603280] |
WOS关键词 | CHLOROGENIC ACID PROTECTS ; GENIPIN PROTECTS ; ALPHA-NAPHTHYLISOTHIOCYANATE ; ISCHEMIA/REPERFUSION INJURY ; RHUBARB ANTHRAQUINONES ; D-GALACTOSAMINE ; GENIPOSIDE ; ANTIOXIDANT ; HEPATOTOXICITY ; INFLAMMATION |
WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
语种 | 英语 |
出版者 | ELSEVIER IRELAND LTD |
WOS记录号 | WOS:000465049300019 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/290002] |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Pan, Guoyu; Huang, Chenggang |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Materia Med, 501 Hai Ke Rd Zhangjiang, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Tian, Xiaoting,Liu, Huan,Qiao, Shida,et al. Exploration of the hepatoprotective chemical base of an orally administered herbal formulation (YCHT) in normal and CCl4-intoxicated liver injury rats. Part 2: Hepatic disposition in vivo and hepatoprotective activity in vitro[J]. JOURNAL OF ETHNOPHARMACOLOGY,2019,236:161-172. |
APA | Tian, Xiaoting.,Liu, Huan.,Qiao, Shida.,Yin, Hao.,Chen, Mingcang.,...&Huang, Chenggang.(2019).Exploration of the hepatoprotective chemical base of an orally administered herbal formulation (YCHT) in normal and CCl4-intoxicated liver injury rats. Part 2: Hepatic disposition in vivo and hepatoprotective activity in vitro.JOURNAL OF ETHNOPHARMACOLOGY,236,161-172. |
MLA | Tian, Xiaoting,et al."Exploration of the hepatoprotective chemical base of an orally administered herbal formulation (YCHT) in normal and CCl4-intoxicated liver injury rats. Part 2: Hepatic disposition in vivo and hepatoprotective activity in vitro".JOURNAL OF ETHNOPHARMACOLOGY 236(2019):161-172. |
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