Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment

doi:10.1002/pmic.201800471

	Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment
	Gao, Yan 1,2; Lee, Hyojin 3,4; Kwon, Oh Kwang 1,2; Cheng, Zhongyi 5; Tan, Minjia 6; Kim, Ki-Tae 3,4; Lee, Sangkyu 1,2
刊名	PROTEOMICS
	2019-05-01
卷号	19 期号:9 页码:4
关键词	D rerio histidine phosphorylation TiO2 affinity chromatography
ISSN号	1615-9853
DOI	10.1002/pmic.201800471
通讯作者	Kim, Ki-Tae(ktkim@seoultech.ac.kr) ; Lee, Sangkyu(sangkyu@knu.ac.kr)
英文摘要	Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hidden vertebrate phosphoproteome, this report presents a global phosphorylation analysis of Danio rerio (zebrafish) larvae. Phosphopeptide enrichment is performed using a TiO2 affinity technique. A total of 68 unique phosphohistidine sites are detected on 63 proteins among 1076 unique phosphosites on 708 proteins. Data are available via ProteomeXchange with identifier PXD012735. This report provides the first phosphohistidine dataset obtained from zebrafish.
资助项目	National Research Foundation of Korea(NRF) - Korea government[NRF-2018R1D1A1A02043591] ; Korea Ministry of Environment (MOE) as the Environmental Health Action Program[2016001370001]
WOS关键词	GLOBAL PROTEOMIC ANALYSIS ; PROTEIN ; PHOSPHORYLATION ; PHOSPHOHISTIDINE ; ACETYLATION ; PROKARYOTES ; RESOURCE ; KINASES
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000471337400002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/289515]
专题	中国科学院上海药物研究所
通讯作者	Kim, Ki-Tae; Lee, Sangkyu
作者单位	1.Kyungpook Natl Univ, Plus KNU Multiom Based Creat Drug Res Team, Coll Pharm, BK21, Daegu 41566, South Korea 2.Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Daegu 41566, South Korea 3.Seoul Natl Univ Sci & Technol, Dept Energy & Environm Engn, Seoul 01811, South Korea 4.Seoul Natl Univ Sci & Technol, Dept Environm Engn, Seoul 01811, South Korea 5.Jingjie PTM BioLab Hangzhou Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yan,Lee, Hyojin,Kwon, Oh Kwang,et al. Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment[J]. PROTEOMICS,2019,19(9):4.
APA	Gao, Yan.,Lee, Hyojin.,Kwon, Oh Kwang.,Cheng, Zhongyi.,Tan, Minjia.,...&Lee, Sangkyu.(2019).Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment.PROTEOMICS,19(9),4.
MLA	Gao, Yan,et al."Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment".PROTEOMICS 19.9(2019):4.