Uncovering kappa-opioid receptor agonist-induced PAK1/2 phosphorylation by quantitative phosphoproteomics

doi:10.1016/j.bbrc.2019.06.111

	Uncovering kappa-opioid receptor agonist-induced PAK1/2 phosphorylation by quantitative phosphoproteomics
	Wu, Jianhong 1,2,3; Jiang, Qinghui 2,3,4; Zhu, Hongwen 2,3; Zhou, Yanting 2,3; Lu, Dayun 2,3,4; Liu, Xing 2,3; Chen, Xiangling 2,3,4; Chen, Jie 2,3; Wang, Yujun 2,3,4; Liu, Jinggen 2,3,4
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2019-08-13
卷号	516 期号:1 页码:320-326
关键词	Dimethyl labeling Kappa-opioid receptor Kinase-substrate network p21-activated kinases Quantitative phosphoproteomics
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2019.06.111
通讯作者	Huang, Ruimin(rmhuang@simm.ac.cn) ; Zhou, Hu(zhouhu@simm.ac.cn)
英文摘要	Kappa-opioid receptor (KOR) is a member of G-protein coupled receptors (GPCRs) expressed in serotonergic neurons and neuronal terminals. The involvement of KOR ligands in nociception, diuresis, emotion, cognition, and immune system has been extensively studied. Omics-based methods are preferable to understand the signaling cascade after KOR activation in a systematic manner. In this study, an in-depth quantitative phosphoproteomic analysis resulted in 305 phosphosites, which were significantly changed in three KOR-overexpressed cells upon treatment with two KOR agonists. The subsequent substrate-kinase prediction analysis revealed that 18 potential kinases might be activated under stimulation of the agonists. We found that phosphorylation of PAK1/2 (p21-activated kinase 1/2) was induced by KOR agonists, resulting in reduced actin stress fibers and cytoskeletal reorganization. In summary, this quantitative phosphoproteomics-based research studied the downstream phosphorylation events upon KOR activation, which may shed light on the investigations of KOR signaling pathway and targeted therapy for KOR-related diseases. (C) 2019 Elsevier Inc. All rights reserved.
资助项目	National Key Research and Development Program from the Ministry of Science and Technology of China[2017YFC1700200] ; National Natural Science Foundation of China[21375138] ; National Natural Science Foundation of China[31570830] ; National Natural Science Foundation of China[91859106] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program, China[2018ZX09711002] ; One Hundred Talent Program of Chinese Academy of Sciences ; Innovation Project of Instrument and Equipment Function Development from the Bureau of Goods, Chinese Academy of Sciences[YZ201542]
WOS关键词	SYSTEM
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000476580800048
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/289133]
专题	中国科学院上海药物研究所
通讯作者	Huang, Ruimin; Zhou, Hu
作者单位	1.Shanghai Univ, Sch Life Sci, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Shanghai Univ Tradit Chinese Med, E Inst, Shanghai Municipal Educ Comm, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Wu, Jianhong,Jiang, Qinghui,Zhu, Hongwen,et al. Uncovering kappa-opioid receptor agonist-induced PAK1/2 phosphorylation by quantitative phosphoproteomics[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,516(1):320-326.
APA	Wu, Jianhong.,Jiang, Qinghui.,Zhu, Hongwen.,Zhou, Yanting.,Lu, Dayun.,...&Zhou, Hu.(2019).Uncovering kappa-opioid receptor agonist-induced PAK1/2 phosphorylation by quantitative phosphoproteomics.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,516(1),320-326.
MLA	Wu, Jianhong,et al."Uncovering kappa-opioid receptor agonist-induced PAK1/2 phosphorylation by quantitative phosphoproteomics".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 516.1(2019):320-326.