Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal

doi:10.1016/j.bbr.2019.112008

	Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal
	Guo, Liu-bin 1; Yu, Chuan 2,3; Ling, Qing-lan 2,3; Fu, Yu 2,3; Wang, Yu-jun 2,3; Liu, Jing-gen 2,3
刊名	BEHAVIOURAL BRAIN RESEARCH
	2019-10-17
卷号	372 页码:8
关键词	Morphine withdrawal Aversive memories Proteomics Synaptic plasticity Amygdala Dorsal hippocampus
ISSN号	0166-4328
DOI	10.1016/j.bbr.2019.112008
通讯作者	Liu, Jing-gen(jgliu@mail.shcnc.ac.cn)
英文摘要	Addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations, encoded by changes of gene expression. We previously demonstrated that the changes in synaptic plasticity were required for the formation of aversive memories associated with morphine withdrawal. However, the proteins involved in synaptic plasticity and aversive memory formation have not been well explored. In the present study, we employed a two-dimensional gel electrophoresis (2-DE)-based proteomic technique to detect the changes of protein expression in the nucleus accumbens, amygdala and dorsal hippo campus of the rats that had developed conditioned morphine withdrawal. We found that twenty-three proteins were significantly altered in the amygdala and dorsal hippocampus after conditioned morphine withdrawal. These proteins can be classified into multiple categories, such as energy metabolism, signal transduction, synaptic transmission, cytoskeletal proteins, chaperones, and protein metabolism according to their biological functions. Eight proteins related to synaptic plasticity were further confirmed by western blot analysis. It is very likely that these identified proteins may contribute to conditioned morphine withdrawal-induced neural plasticity and aversive memory formation. Thus, our work will help understand the potential mechanism associated with generation of drug withdrawal memories.
资助项目	National Natural Science Foundation of China[81671322]
WOS关键词	ACTIN REARRANGEMENTS ; TWINFILIN ISOFORMS ; OPIOID RECEPTOR ; DRUG-WITHDRAWAL ; NMDA RECEPTORS ; EXPRESSION ; MEMORY ; CALCINEURIN ; PLASTICITY ; ADDICTION
WOS研究方向	Behavioral Sciences ; Neurosciences & Neurology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000478709300011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/289021]
专题	中国科学院上海药物研究所
通讯作者	Liu, Jing-gen
作者单位	1.China Pharmaceut Univ, Sch Basic Med & Clin Pharm, Nanjing 210009, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Collaborat Innovat Ctr Brain Sci, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Guo, Liu-bin,Yu, Chuan,Ling, Qing-lan,et al. Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal[J]. BEHAVIOURAL BRAIN RESEARCH,2019,372:8.
APA	Guo, Liu-bin,Yu, Chuan,Ling, Qing-lan,Fu, Yu,Wang, Yu-jun,&Liu, Jing-gen.(2019).Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal.BEHAVIOURAL BRAIN RESEARCH,372,8.
MLA	Guo, Liu-bin,et al."Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal".BEHAVIOURAL BRAIN RESEARCH 372(2019):8.