Enantioselective determination of ketamine in dog plasma by chiral liquid chromatography-tandem mass spectrometry

doi:10.1002/bmc.4578

	Enantioselective determination of ketamine in dog plasma by chiral liquid chromatography-tandem mass spectrometry
	Li, Ranran 1,2; Zhong, Kan 3; Jiang, Jinfang 3; Zhan, Yuan 3; Chen, Xiaoyan 2
刊名	BIOMEDICAL CHROMATOGRAPHY
	2019-09-01
卷号	33 期号:9 页码:8
关键词	chiral stationary phases enantioselective LC-MS MS ketamine stereoselective pharmacokinetics
ISSN号	0269-3879
DOI	10.1002/bmc.4578
通讯作者	Chen, Xiaoyan(xychen@simm.ac.cn)
英文摘要	Ketamine is an N-methyl-d-aspartate receptor antagonist that is usually used clinically as a racemic mixture. Its two enantiomers exhibit different pharmacological activities. To determine whether the enantiomers have different pharmacokinetic profiles, a chiral liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of ketamine enantiomers in dog plasma. The enantiomers of ketamine were extracted from 50 mu L of plasma by methyl tert-butyl ether. Adequate chromatographic retention and baseline resolution of the enantiomers were achieved within a runtime of 5 min on a chiral column coated with polysaccharide derivatives, using a gradient mobile phase of acetonitrile and 10 mm ammonium bicarbonate aqueous solution. Ketamine enantiomers were detected by mass spectrometry with multiple reaction monitoring mode using the transitions of m/z 238.3 -> 125.9 for the analytes and m/z 237.1 -> 194.1 for carbamazepine (internal standard). The method was linear over the concentration range from 0.5 to 500 ng/mL for each enantiomer. The lower limit of quantification (LLOQ) for each enantiomer was 0.5 ng/mL. The intra- and inter-day precision was R- and S-ketamine, respectively. The accuracy was 92.9-110.4% for R-ketamine and 99.8-102.4% for S-ketamine. The method was successfully applied to characterize the stereoselective pharmacokinetic profiles of ketamine in beagle dogs.
WOS关键词	LC-MS/MS ; NORKETAMINE ; METABOLITES ; URINE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	WILEY
WOS记录号	WOS:000479085300022
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/288917]
专题	中国科学院上海药物研究所
通讯作者	Chen, Xiaoyan
作者单位	1.Univ Sci & Technol China, Nano Sci & Technol Inst, Suzhou, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Suzhou HQ Biosci Co Ltd, Suzhou, Peoples R China
推荐引用方式 GB/T 7714	Li, Ranran,Zhong, Kan,Jiang, Jinfang,et al. Enantioselective determination of ketamine in dog plasma by chiral liquid chromatography-tandem mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2019,33(9):8.
APA	Li, Ranran,Zhong, Kan,Jiang, Jinfang,Zhan, Yuan,&Chen, Xiaoyan.(2019).Enantioselective determination of ketamine in dog plasma by chiral liquid chromatography-tandem mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,33(9),8.
MLA	Li, Ranran,et al."Enantioselective determination of ketamine in dog plasma by chiral liquid chromatography-tandem mass spectrometry".BIOMEDICAL CHROMATOGRAPHY 33.9(2019):8.