modifiedcitruspectininhibitedbladdertumorgrowththroughdownregulationofgalectin3

doi:10.1038/s41401-018-0004-z

	modifiedcitruspectininhibitedbladdertumorgrowththroughdownregulationofgalectin3
	Fang Tian 1; Liu Dandan 2; Ning Heming 2; Liu Dan 1; Sun Jingya 2; Huang Xiaojing 1; Dong Yu 2; Geng Meiyu 2; Yun Shifeng 1; Yan Jun 1
刊名	actapharmacologicasinica
	2018
卷号	39 期号:12 页码:1885
关键词	CANCER CELLS APOPTOSIS EXPRESSION BINDING PHOSPHORYLATION PROGRESSION STATISTICS TARGET AKT modified citrus pectin urinary bladder cancer galectin-3 xenograft cell survival
ISSN号	1671-4083
DOI	10.1038/s41401-018-0004-z
英文摘要	Modified citrus pectin (MCP) is a carbohydrate enriched complex, which has been implicated in cancer treatment and prevention. However, the effects of MCP on urinary bladder cancer (UBC) are unknown. In this study, MCP was first tested in T24 and J82 human UBC cells and showed the inhibition of cell viability by the sulforhodamine B (SRB) assay. The MCP-treated UBC cells exhibited G(2)/M phase arrest with the decrease of Cyclin B1 and phosphorylated Cdc2. Caspase-3 was also activated, leading to the cleavage of Caspase-3 and PARP. We further explored the possible molecular mechanisms upon MCP treatment in UBC cells. Reduction of galectin-3 was observed and followed with the inactivation of Akt signaling pathway. Of note, galectin-3 knockdown by RNA interference recapitulated the MCP-mediated anti-proliferation, cell cycle arrest and apoptosis. Moreover, oral administration of MCP to the T24 xenograft-bearing nude mice inhibited the tumor growth significantly (P < 0.05). Quantification analysis of immunohistochemistry staining for Ki67 and cleaved Caspase-3 confirmed the decrease of proliferation index (P < 0.05) and the increase of apoptosis index (P < 0.01) in 700 mg/kg MCP-fed UBC xenografts. Using the information from TCGA database, we revealed that the overexpression of galectin-3 was associated with high tumor grade with lymph node metastasis, poor overall survival in UBC patients. Considering the remarkable inhibitory effects of MCP on UBC cell proliferation and survival in vitro and in vivo mainly through galectin-3, which is upregulated in UBCs, MCP may become an attractive agent, as a natural dietary fiber, for prevention and therapy of UBCs.
资助项目	[National Natural Science Foundation of China] ; [Natural Science Foundation for Universities in Jiangsu Province] ; [One Hundred Talent Program of Chinese Academy of Sciences] ; [Research Fund of Institutes for Drug Discovery and Development, Chinese Academy of Sciences] ; [Institutional Technology Service Center of Shanghai Institute of Materia Medica]
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/283925]
专题	中国科学院上海药物研究所
作者单位	1.南京大学 2.中国科学院上海药物研究所
推荐引用方式 GB/T 7714	Fang Tian,Liu Dandan,Ning Heming,et al. modifiedcitruspectininhibitedbladdertumorgrowththroughdownregulationofgalectin3[J]. actapharmacologicasinica,2018,39(12):1885.
APA	Fang Tian.,Liu Dandan.,Ning Heming.,Liu Dan.,Sun Jingya.,...&Huang Ruimin.(2018).modifiedcitruspectininhibitedbladdertumorgrowththroughdownregulationofgalectin3.actapharmacologicasinica,39(12),1885.
MLA	Fang Tian,et al."modifiedcitruspectininhibitedbladdertumorgrowththroughdownregulationofgalectin3".actapharmacologicasinica 39.12(2018):1885.