A comprehensive study of pomegranate flowers polyphenols and metabolites in rat biological samples by high-performance liquid chromatography quadrupole time-of-flight mass spectrometry

doi:10.1016/j.chroma.2019.460472

	A comprehensive study of pomegranate flowers polyphenols and metabolites in rat biological samples by high-performance liquid chromatography quadrupole time-of-flight mass spectrometry
	Yisimayili, Zainaipuguli 1,2,3; Abdulla, Rahima 1; Tian, Qiang 2; Wang, Yangyang 2,3; Chen, Mingcang 2; Sun, Zhaolin 2; Li, Zhixiong 2; Liu, Fang 2; Aisa, Haji Akber 1,3; Huang, Chenggang 2,3
刊名	JOURNAL OF CHROMATOGRAPHY A
	2019-10-25
卷号	1604 页码:13
关键词	Punica granatum L. flowers Polyphenols Ellagitannin Metabolism HPLC-Q-TOF-MS2
ISSN号	0021-9673
DOI	10.1016/j.chroma.2019.460472
通讯作者	Aisa, Haji Akber(haji@ms.xjb.ac.cn) ; Huang, Chenggang(cghsimm@126.com)
英文摘要	Pomegranate flowers is an ancient medicine that has commonly been used to treat various diseases such as diabetes. However, no reports are available on the metabolic profile of pomegranate flowers in vivo. In the present study, with the aid of HPLC-Q-TOF-MS2, 67 compounds were identified in pomegranate flowers extract, including 18 ellagitannins, 14 gallic acid and galloyl derivatives, five anthocyanins and 18 flavonoids. Seven compounds were firstly identified. In vivo, 22 absorbed compounds and 35 metabolites were identified in rat biosamples (urine, feces, plasma and tissues) after orally administered with pomegranate flowers extract. This result showed that not all compounds abundant in pomegranate flowers extract could be absorbed well in plasma and tissues. This finding also suggested a potential correlation between study on metabolic profile of these compounds in vivo and study on strategy of screening bioactivity of the isolates with in vitro cell systems evaluation. Notably, mono-glucuronide conjugated metabolite of ellagitannin compound (corilagin) was firstly identified. In addition, this is first report to identify phase II conjugate metabolites of ellagitannins in vivo after oral administration of ellagitanninsrich extracts (or foods). Thus, characterizing its multiple constitution, absorption and metabolic fate of these compounds in vivo is helpful to better analyze the active components in pomegranate flowers. (C) 2019 The Authors. Published by Elsevier B.V.
资助项目	Science and Technology Service Network Initiative of the Chinese Academy of Sciences[KFJ-STS-QYZD-066]
WOS关键词	PUNICA-GRANATUM ; PHENOLIC-COMPOUNDS ; ALPHA-GLUCOSIDASE ; ELLAGITANNINS ; EXTRACT ; QUANTIFICATION ; IDENTIFICATION ; ANTHOCYANINS ; ACID
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000488303200011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/282663]
专题	中国科学院上海药物研究所
通讯作者	Aisa, Haji Akber; Huang, Chenggang
作者单位	1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, Urumqi 830011, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yisimayili, Zainaipuguli,Abdulla, Rahima,Tian, Qiang,et al. A comprehensive study of pomegranate flowers polyphenols and metabolites in rat biological samples by high-performance liquid chromatography quadrupole time-of-flight mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2019,1604:13.
APA	Yisimayili, Zainaipuguli.,Abdulla, Rahima.,Tian, Qiang.,Wang, Yangyang.,Chen, Mingcang.,...&Huang, Chenggang.(2019).A comprehensive study of pomegranate flowers polyphenols and metabolites in rat biological samples by high-performance liquid chromatography quadrupole time-of-flight mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1604,13.
MLA	Yisimayili, Zainaipuguli,et al."A comprehensive study of pomegranate flowers polyphenols and metabolites in rat biological samples by high-performance liquid chromatography quadrupole time-of-flight mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1604(2019):13.