Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5 alpha-Reductase

doi:10.1038/s41598-019-56145-z

	Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5 alpha-Reductase
	Liu, Jiakuan 2,3; Fang, Tian 1; Li, Meiqian 2,3; Song, Yuting 2,3; Li, Junzun 2,3; Xue, Zesheng 2,3; Li, Jiaxuan 2,3; Bu, Dandan 2,3; Liu, Wei 2,3; Zeng, Qinghe 4,5
刊名	SCIENTIFIC REPORTS
	2019-12-23
卷号	9 页码:10
ISSN号	2045-2322
DOI	10.1038/s41598-019-56145-z
通讯作者	Yun, Shifeng(yunshifeng1@163.com) ; Huang, Ruimin(rmhuang@simm.ac.cn) ; Yan, Jun(yanjun@nju.edu.cn)
英文摘要	Benign prostatic hyperplasia (BPH) is one of the most common diseases in the urinary system of elderly men. Pao extract is an herbal preparation of the bark of the Amazon rainforest tree Pao Pereira (Geissospermum vellosii), which was reported to inhibit prostate cancer cell proliferation. Herein we investigated the therapeutic potential of Pao extract against BPH development in a testosterone-induced BPH rat model. The administration of testosterone induced the prostate enlargement, compared with the sham operated group with vehicle treatment. The BPH/Pao group showed reduced prostate weight comparable with BPH/finasteride group. Notably, Pao treatment did not significantly reduce body weights and sperm number of rats, compared with the control group. Furthermore, Pao extract treatment reduced the proliferative index in prostate glands and testosterone-induced expression levels of AR, as well as androgen-associated proteins such as SRD5A1 and PSA. Moreover, Pao extract and its active component, flavopereirine, induced cytotoxicity on human prostate epithelial RWPE-1 cells in a dose- and time- dependent manner with G2/M arrest. Consistently, Pao extract and flavopereirine suppressed the expression levels of SRD5A1, AR and PSA, respectively. Together, these data demonstrated that Pao extract suppresses testosterone-induced BPH development through inhibiting AR activity and expression, and suggested that Pao extract may be a promising and relative safe agent for BPH.
资助项目	National Natural Science Foundation of China[81771890] ; National Natural Science Foundation of China[81672873] ; One Hundred Talent Program of Chinese Academy of Sciences ; Beljanski Foundation ; 'Personalized Medicines-Molecular Signature - based Drug Discovery and Development', Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020108] ; Military Laboratory Animal Fund[SYDW[2017]15] ; Postgraduate Research & Practice Innovation Program of Jiangsu Province[KYCX18_0042]
WOS关键词	5-ALPHA REDUCTASE INHIBITORS ; ERECTILE DYSFUNCTION ; ALPHA-BLOCKERS ; STEM BARK ; CANCER ; ALKALOIDS ; BPH ; DIHYDROTESTOSTERONE ; FINASTERIDE ; PROGRESSION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000508904100012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/282412]
专题	中国科学院上海药物研究所
通讯作者	Yun, Shifeng; Huang, Ruimin; Yan, Jun
作者单位	1.Nanjing Univ, Jinling Hosp, Dept Comparat Med, Sch Med, Nanjing 210002, Peoples R China 2.Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Model Anim Res Ctr, Nanjing 210061, Peoples R China 3.Nanjing Univ, MOE Key Lab Model Anim Dis Study, Model Anim Res Ctr, Nanjing 210061, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Nanjing Univ, Sch Chem & Life Sci, Dept Biosci & Bioengn, Jinling Coll, Nanjing 210061, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jiakuan,Fang, Tian,Li, Meiqian,et al. Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5 alpha-Reductase[J]. SCIENTIFIC REPORTS,2019,9:10.
APA	Liu, Jiakuan.,Fang, Tian.,Li, Meiqian.,Song, Yuting.,Li, Junzun.,...&Yan, Jun.(2019).Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5 alpha-Reductase.SCIENTIFIC REPORTS,9,10.
MLA	Liu, Jiakuan,et al."Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5 alpha-Reductase".SCIENTIFIC REPORTS 9(2019):10.