Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry

doi:10.1016/j.sbi.2019.06.007

	Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry
	Zheng, Jie 1,2,3; Strutzenberg, Timothy 2,3; Pascal, Bruce D.2,3,4; Griffin, Patrick R.2,3
刊名	CURRENT OPINION IN STRUCTURAL BIOLOGY
	2019-10-01
卷号	58 页码:305-313
ISSN号	0959-440X
DOI	10.1016/j.sbi.2019.06.007
通讯作者	Griffin, Patrick R.(pgriffin@scripps.edu)
英文摘要	Proteins are not rigid bodies under their physiological conditions. Here we discuss a solution-phase structural proteomics technique, hydrogen deuterium exchange coupled with mass spectrometry (HDX-MS), as a means to study protein dynamics, which can complement other structural approaches. We outline the background theory and highlight the utility of HDX-MS measurements in two case studies involving a nuclear receptor and an innate immunity receptor. We also discuss emerging software advances for improving data analysis and three-dimensional visualization.
WOS关键词	HYDROGEN-DEUTERIUM EXCHANGE ; SINGLETON-MERTEN SYNDROME ; TIME WINDOW ; CROSS-LINKING ; RIG-I ; MS ; TECHNOLOGY ; EXPANSION ; PEPTIDES ; DOMAIN
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	CURRENT BIOLOGY LTD
WOS记录号	WOS:000494891800037
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/281831]
专题	中国科学院上海药物研究所
通讯作者	Griffin, Patrick R.
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Scripps Res Inst, Dept Mol Med, Jupiter, FL 33458 USA 3.Scripps Res Inst, Dept Integrat Struct & Computat Biol, Jupiter, FL 33458 USA 4.Omics Informat LLC, Honolulu, HI 96813 USA
推荐引用方式 GB/T 7714	Zheng, Jie,Strutzenberg, Timothy,Pascal, Bruce D.,et al. Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry[J]. CURRENT OPINION IN STRUCTURAL BIOLOGY,2019,58:305-313.
APA	Zheng, Jie,Strutzenberg, Timothy,Pascal, Bruce D.,&Griffin, Patrick R..(2019).Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry.CURRENT OPINION IN STRUCTURAL BIOLOGY,58,305-313.
MLA	Zheng, Jie,et al."Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry".CURRENT OPINION IN STRUCTURAL BIOLOGY 58(2019):305-313.