Quantitative analysis of fourteen bufadienolides in Venenum Bufonis crude drug and its Chinese patent medicines by ultra-high performance liquid chromatography coupled with tandem mass spectrometry

doi:10.1016/j.jep.2019.112490

	Quantitative analysis of fourteen bufadienolides in Venenum Bufonis crude drug and its Chinese patent medicines by ultra-high performance liquid chromatography coupled with tandem mass spectrometry
	Wei, Wen-long; An, Ya-ling; Zhang, Yan-zhi; Li, Zhen-wei; Zhou, Yang; Lei, Min; Zhang, Jian-qing; Qu, Hua; Da, Juan; Wu, Wan-ying
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2020-04-06
卷号	251 页码:8
关键词	Venenum Bufonis Multi-component quantification Quality evaluation UHPLC-QqQ-MS/MS
ISSN号	0378-8741
DOI	10.1016/j.jep.2019.112490
通讯作者	Wu, Wan-ying(wanyingwu@simm.ac.cn) ; Guo, De-an(daguo@simm.ac.cn)
英文摘要	Ethnopharmacological relevance: Venenum Bufonis, a product of the secretions of Bufo gargarizans Cantor or B. melanostictus Schneider, possessed an array of pharmacological activities, such as cardiotonic, anti-tumor, antinociceptive, anti-inflammatory, anesthetic and antimicrobial activities. However, there were few efficient methods for quality evaluation of Venenum Bufonis medicinal materials and its related Chinese patent medicines. Aim of the study: To establish an effective method for quality assessment of crude drugs and Chinese proprietary medicines of Venenum Bufonis, and explore the relationship of primary compounds - target - pathway - disease through a series of network databases. Materials and methods: An ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-QqQ-MS/MS) method was developed and validated to simultaneously determine 14 bufadienolides for quantitative analysis of 71 batches of crude drugs and 20 kinds of Chinese patent medicines of Venenum Bufonis. Multiple reaction monitoring with good specificity and accuracy was applied to monitor the 14 bufadienolides in positive mode. Results: The methodology was validated with good specificity, precision, stability, repeatability and recovery. The low limits of quantification were in the range of 0.1-2.7 ng/mL. The relative standard deviation values for intra- and inter-day precisions ranged from 0.98% to 6.3% and from 2.39% to 6.76%, respectively. The recovery was varied from 87.78% to 110.57% for crude drugs and 88.32%-100.96% for Chinese proprietary medicine (Shexiang Baoxin Pill). The contents of 14 analytes in 71 batches of crude drugs and 20 sorts of Chinese proprietary medicines were procured, the results showed that the contents of crude drugs collected from the market exhibited great variations. Furthermore, 13 batches of crude drugs were identified as counterfeit with no bufadienolides detected. In addition, the total contents of bufadienolides in the same drug showed great difference among products from various manufacturers or brands. Subsequently, 9 bufadienolides with the higher contents were applied to screen the anti-tumor effect by network pharmacology, and 8 pathways which had prior correlation with bufadienolides were disclosed. Conclusion: This method could be used for quality assessment of crude drugs and Chinese patent medicines of Venenum Bufonis, and the data could be served as the fundamental basis for drug research and development of Venenum Bufonis.
资助项目	National Natural Science Foundation of China[81530095] ; National Natural Science Foundation of China[81673591] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020348] ; National Standardization of Traditional Chinese Medicine Project[ZYBZH-K-LN-01] ; Science and Technology Commission Foundation of Shanghai[15DZ0502800]
WOS关键词	CANCER ; APOPTOSIS ; PI3K/AKT ; CHANSU
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000515196000023
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/281320]
专题	中国科学院上海药物研究所
通讯作者	Wu, Wan-ying; Guo, De-an
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Haike Rd 501, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wei, Wen-long,An, Ya-ling,Zhang, Yan-zhi,et al. Quantitative analysis of fourteen bufadienolides in Venenum Bufonis crude drug and its Chinese patent medicines by ultra-high performance liquid chromatography coupled with tandem mass spectrometry[J]. JOURNAL OF ETHNOPHARMACOLOGY,2020,251:8.
APA	Wei, Wen-long.,An, Ya-ling.,Zhang, Yan-zhi.,Li, Zhen-wei.,Zhou, Yang.,...&Guo, De-an.(2020).Quantitative analysis of fourteen bufadienolides in Venenum Bufonis crude drug and its Chinese patent medicines by ultra-high performance liquid chromatography coupled with tandem mass spectrometry.JOURNAL OF ETHNOPHARMACOLOGY,251,8.
MLA	Wei, Wen-long,et al."Quantitative analysis of fourteen bufadienolides in Venenum Bufonis crude drug and its Chinese patent medicines by ultra-high performance liquid chromatography coupled with tandem mass spectrometry".JOURNAL OF ETHNOPHARMACOLOGY 251(2020):8.