c10orf12modulatesprc2histonemethyltransferaseactivityandh3k27me3levels

doi:10.1038/s41401-019-0247-3

	c10orf12modulatesprc2histonemethyltransferaseactivityandh3k27me3levels
	Shi Yi; Ma Honglei; Zhuang Youwen; Wang Xiaoxi; Jiang Yi; Eric Xu H
刊名	actapharmacologicasinica
	2019
卷号	40 期号:11 页码:1457
关键词	REPRESSIVE COMPLEX 2 GENOMIC TARGETS POLYCOMB PROTEIN CHROMATIN RECRUITMENT JARID2 DIFFERENTIATION BINDING METHYLATION PRC2 C10ORF12 H3K27me3 histone methyltransferase epigenetic regulation histone modification
ISSN号	1671-4083
DOI	10.1038/s41401-019-0247-3
英文摘要	The polycomb repressive complex 2 (PRC2) catalyzes the methylation of histone H3 on lysine 27 (H3K27) to generate trimethyl-H3K27 (H3K27me3) marks, thereby leading to a repressive chromatin state that inhibits gene expression. C10ORF12 was recently identified as a novel PRC2 interactor. Here, we show that C10ORF12 specifically interacts with PRC2 through its middle region (positions 619-718). C10ORF12 significantly enhances the histone methyltransferase activity of PRC2 in vitro and dramatically increases the total H3K27me3 levels in HeLa cells. C10ORF12 also antagonizes Jarid2, which is an auxiliary factor of the PRC2.2 subcomplex, to promote increased H3K27me3 levels in HeLa cells. Moreover, C10ORF12 alters the substrate preference of PRC2. These results indicate that C10ORF12 functions as a positive regulator of PRC2 and facilitates PRC2-mediated H3K27me3 modification of chromatin. These findings provide new insight into the roles of C10ORF12 in regulating the activity of the PRC2 complex.
资助项目	[Strategic Priority Research Program of CAS] ; [National Natural Science Foundation of China] ; [Outstanding Young Scientist Foundation of CAS] ; [Youth Innovation Promotion Association of CAS]
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280672]
专题	中国科学院上海药物研究所
作者单位	中国科学院上海药物研究所
推荐引用方式 GB/T 7714	Shi Yi,Ma Honglei,Zhuang Youwen,et al. c10orf12modulatesprc2histonemethyltransferaseactivityandh3k27me3levels[J]. actapharmacologicasinica,2019,40(11):1457.
APA	Shi Yi,Ma Honglei,Zhuang Youwen,Wang Xiaoxi,Jiang Yi,&Eric Xu H.(2019).c10orf12modulatesprc2histonemethyltransferaseactivityandh3k27me3levels.actapharmacologicasinica,40(11),1457.
MLA	Shi Yi,et al."c10orf12modulatesprc2histonemethyltransferaseactivityandh3k27me3levels".actapharmacologicasinica 40.11(2019):1457.