Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NFκB signaling

doi:10.1186/s12906-020-02943-2

	Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NFκB signaling
	Dong,Yu 1,6; Liu,Jiakuan 7; Xue,Zesheng 7; Sun,Jingya 1; Huang,Zhengnan 5; Jing,Yifeng 5; Han,Bangmin 5; Shen,Bing 5; Yan,Jun 3,4; Huang,Ruimin 1,2
刊名	BMC Complementary Medicine and Therapies
	2020-05-16
卷号	20 期号:1
关键词	Pao Pereira extract BPH NFκB Inflammation Extracellular matrix
DOI	10.1186/s12906-020-02943-2
通讯作者	Shen,Bing(shenbingyishi@163.com) ; Yan,Jun(yan_jun@fudan.edu.cn) ; Huang,Ruimin(rmhuang@simm.ac.cn)
英文摘要	AbstractBackgroundOur previous study revealed the extract from the bark of an Amazonian tree Pao Pereira can suppress benign prostatic hyperplasia (BPH) in a rat model. Herein, we examined its inhibitory effects on human BPH cells and dissect its molecular mechanism.MethodsWe applied Pao extract to human BPH epithelial BPH-1 and prostate myofibroblast WPMY-1 cells. Cell viability, apoptosis and immunoblotting were performed, followed by gene expression profiling and gene set enrichment analysis (GSEA) to detect the differentially expressed genes and signaling pathway induced by Pao extract. Human ex vivo BPH explant organ culture was also used to examine the effects of Pao extract on human BPH tissues.ResultsPao extract treatment inhibited viability and induced apoptosis in human BPH-1 and WPMY-1 cells. Gene expression profiling and the following validation indicated that the expression levels of pro-apoptotic genes (eg. PCDC4, CHOP and FBXO32) were induced by Pao extract in both two cell lines. GSEA further revealed that Pao extract treatment was negatively associated with the activation of NFκB signaling. Pao extract suppressed the transcriptional activity of NFκB and down-regulated its target genes involved in inflammation (CXCL5, CXCL6 and CXCL12) and extracellular matrix (ECM) remodeling (HAS2, TNC and MMP13) in both cultured cells and human ex vivo BPH explants.ConclusionIn both BPH epithelial and stromal cells, Pao extract induces apoptosis by upregulating the pro-apoptotic genes and inhibiting the inflammation-associated NFκB signaling via reducing phosphorylation of NFκB subunit RelA. Our data suggest that Pao extract may be a promising phytotherapeutic agent for BPH.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/S12906-020-02943-2
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280646]
专题	中国科学院上海药物研究所
通讯作者	Shen,Bing; Yan,Jun; Huang,Ruimin
作者单位	1.Chinese Academy of Sciences; Shanghai Institute of Materia Medica 2.University of Chinese Academy of Sciences 3.Model Animal Research Center of Nanjing University; MOE Key Laboratory of Model Animals for Disease Study 4.Fudan University; Department of Laboratory Animal Science 5.Shanghai Jiaotong University; Department of Urology, Shanghai General Hospital 6.Shanghai University 7.Model Animal Research Center of Nanjing University
推荐引用方式 GB/T 7714	Dong,Yu,Liu,Jiakuan,Xue,Zesheng,et al. Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NFκB signaling[J]. BMC Complementary Medicine and Therapies,2020,20(1).
APA	Dong,Yu.,Liu,Jiakuan.,Xue,Zesheng.,Sun,Jingya.,Huang,Zhengnan.,...&Huang,Ruimin.(2020).Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NFκB signaling.BMC Complementary Medicine and Therapies,20(1).
MLA	Dong,Yu,et al."Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NFκB signaling".BMC Complementary Medicine and Therapies 20.1(2020).