Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus

doi:10.1111/mmi.14519

	Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus
	Yang, Nana 1,5; Cao, Qiao 1,6; Hu, Shuyang 1,5; Xu, Chenchen 1,5; Fan, Ke 1,5; Chen, Feifei 1,6; Yang, Cai-Guang 1,5; Liang, Haihua 6; Wu, Min 2; Bae, Taeok 3
刊名	MOLECULAR MICROBIOLOGY
	2020-05-08
页码	20
关键词	ClpXP microbial interspecies interactions proteolysis Pseudomonas aeruginosa quorum sensing
ISSN号	0950-382X
DOI	10.1111/mmi.14519
通讯作者	Lan, Lefu(llan@simm.ac.cn)
英文摘要	Intracellular protein degradation is essential for the survival of all organisms, but its role in interspecies interaction is unknown. Here, we show that the ClpXP protease of Pseudomonas aeruginosa suppresses its antimicrobial activity against Staphylococcus aureus, a common pathogen co-isolated with P. aeruginosa from polymicrobial human infections. Using proteomic, biochemical, and molecular genetic approaches, we found that this effect is due to the inhibitory effects of ClpXP on the quorum sensing (QS) of P. aeruginosa, mainly by degrading proteins (e.g., PhnA, PhnB, PqsR, and RhlI) which are critical for the production of QS signal molecules PQS and C4-HSL. We provide evidence that co-culturing with S. aureus induces a decrease in the activity of ClpXP in P. aeruginosa, an effect which was also achieved by the treatment of P. aeruginosa with N-acetylglucosamine (GlcNAc), a widespread chemical present on the surface of diverse cell types from bacteria to humans. These findings extend the range of biological events governed by proteolytic machinery to microbial community structure, thus also suggesting that a chemical-induced alteration of protein homeostasis is a mechanism for interspecies interactions.
资助项目	National Natural Science Foundation of China[31670136] ; National Natural Science Foundation of China[31870127] ; National Natural Science Foundation of China[81861138047] ; National Natural Science Foundation of China[81661138004] ; Ministry of Science and Technology of the People's Republic of China[2016YFA0501503] ; State Key Laboratory of Drug Research[SIMM2003ZZ-03] ; Science and Technology Commission of Shanghai Municipality[19JC1416400]
WOS关键词	HOST-RANGE PLASMIDS ; REGULATED PROTEOLYSIS ; VIRULENCE FACTORS ; GENE-EXPRESSION ; CLPP ; BACTERIA ; TOLERANCE ; PROTEASES ; REVEALS ; RHLR
WOS研究方向	Biochemistry & Molecular Biology ; Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000530928200001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280607]
专题	中国科学院上海药物研究所
通讯作者	Lan, Lefu
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 2.Univ North Dakota, Dept Biomed Sci, Grand Forks, ND USA 3.Indiana Univ, Sch Med Northwest, Dept Microbiol & Immunol, Gary, IN USA 4.Shanghai Inst Food & Drug Control, NMPA Key Lab Testing Technol Pharmaceut Microbiol, Shanghai, Peoples R China 5.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 6.Northwest Univ, Coll Life Sci, Xian, Peoples R China
推荐引用方式 GB/T 7714	Yang, Nana,Cao, Qiao,Hu, Shuyang,et al. Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus[J]. MOLECULAR MICROBIOLOGY,2020:20.
APA	Yang, Nana.,Cao, Qiao.,Hu, Shuyang.,Xu, Chenchen.,Fan, Ke.,...&Lan, Lefu.(2020).Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus.MOLECULAR MICROBIOLOGY,20.
MLA	Yang, Nana,et al."Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus".MOLECULAR MICROBIOLOGY (2020):20.