IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells

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	IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells
	Zhang Ke; Zhao WeiDong; Li Qiang; Fang WenGang; Zhu Li; Chen YuHua
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2009
卷号	36 期号:4 页码:417-423
关键词	PH TRAFFICKING PENETRATION EXPRESSION PHAGOSOME BARRIER ibeT Escherichia coli K1 HBMEC lysosome escape
ISSN号	1000-3282
其他题名	IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells
英文摘要	Escherichia coli is the most common gram-negative organism causing neonatal meningitis. In order to characterize the role of the pathogenicity island gene ibeT of Escherichia coli K1 in the pathogenesis of neonatal meningitis, an ibeT gene isogenic in-frame deletion mutant strain was constructed. Intracellular survival assay in human brain microvascular endothelial cells (HBMECs) showed that the deletion of ibeT inhibited the growth of Escherichia colt K1 within HBMECs. Confocal microscopy analysis showed that much more ibeT mutant remained in lysosomes of HBMECs compared with wild type Escherichia coli K1 after bacteria invasion into HBMECs. Transmission electron micrographs further showed that ibeT mutant strain was failed to disrupt the Escherichia coli containing vacuole (ECV) and escape into the cytoplasm. Furthermore, cytoplasmic buffering capacities of ibeT mutant were lower than wild type Escherichia coli K1 at pH 6.5 and pH 6.0. These results suggested that the expression of ibeT in Escherichia coli K1 contributed to ECV membrane degradation and subsequent escape from lysosomes into the cytoplasm for replication after Escherichia coli K1 invasion into HBMECs.
资助项目	[National Natural Science Foundation of China] ; [Doctoral Seeding Fund of Liaoning Province]
语种	英语
CSCD记录号	CSCD:3616629
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/143014]
专题	金属研究所_中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhang Ke,Zhao WeiDong,Li Qiang,et al. IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2009,36(4):417-423.
APA	Zhang Ke,Zhao WeiDong,Li Qiang,Fang WenGang,Zhu Li,&Chen YuHua.(2009).IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,36(4),417-423.
MLA	Zhang Ke,et al."IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 36.4(2009):417-423.