Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells

doi:10.1007/s40195-020-01158-1

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	Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells
	Zhang, Ji; Ren, Ling; Yang, Ke
刊名	ACTA METALLURGICA SINICA-ENGLISH LETTERS
	2020-11-11
页码	7
关键词	Cytotoxicity Ti– 6Al– 4V– 5Cu alloy Fabrication Biocompatibility
ISSN号	1006-7191
DOI	10.1007/s40195-020-01158-1
通讯作者	Yang, Ke(kyang@imr.ac.cn)
英文摘要	The aim of this work was to study the cytotoxicity of Ti-6Al-4V-5Cu (TC4-Cu) alloy for dental applications and evaluate the cell viability of different fabricated TC4-Cu alloys in contact with MC3T3-E1 cells in vitro. Ti-6Al-4V (TC4) alloy was used as a negative control to evaluate the cytotoxicity level of TC4-Cu alloy so as to provide basic support for the dental clinical application. Control group TC4 and experimental group TC4-Cu with different fabrications were incubated in the cell culture medium. The absorbance value of mouse osteoblast MC3T3-E1 cells was detected by CCK-8 assay after 1, 3 and 7 days, respectively. The relative proliferation rate of cells was calculated, and then the toxicity level was valued for each group. Cell morphology on the surface was also studied by observing the cytoskeleton through F-actin filament staining. The experimental results showed that the absorbance values for the experimental and the negative control groups were not same at different time points. Compared with different fabricated TC4-Cu alloys, the annealing-TC4-Cu alloy showed much better biocompatibility. The mouse osteoblast MC3T3-E1 cells cultured with annealing-TC4-Cu, rolling-TC4-Cu and solution + aging-TC4-Cu have no toxic effects, and these alloys could promote the proliferation of mouse osteoblasts.
资助项目	National Natural Science Foundation of China[51631009]
WOS研究方向	Metallurgy & Metallurgical Engineering
语种	英语
出版者	CHINESE ACAD SCIENCES, INST METAL RESEARCH
WOS记录号	WOS:000588588000003
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/141315]
专题	金属研究所_中国科学院金属研究所
通讯作者	Yang, Ke
作者单位	Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ji,Ren, Ling,Yang, Ke. Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells[J]. ACTA METALLURGICA SINICA-ENGLISH LETTERS,2020:7.
APA	Zhang, Ji,Ren, Ling,&Yang, Ke.(2020).Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells.ACTA METALLURGICA SINICA-ENGLISH LETTERS,7.
MLA	Zhang, Ji,et al."Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells".ACTA METALLURGICA SINICA-ENGLISH LETTERS (2020):7.