Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application<bold> </bold>

doi:10.1016/j.bioactmat.2020.04.019

CORC > 金属研究所 > 中国科学院金属研究所

	Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application
	Gao, Peng 2,3,4; Fan, Bo 2; Yu, Xiaoming 1,5; Liu, Wenwen 2; Wu, Jie 2; Shi, Lei 2; Yang, Di 2; Tan, Lili 1; Wan, Peng 1; Hao, Yulin 1
刊名	BIOACTIVE MATERIALS
	2020-09-01
卷号	5 期号:3 页码:680-693
关键词	Porous Ti6Al4V scafflod Surface modification Magnesium coating Osteogenesis Angiogenesis
DOI	10.1016/j.bioactmat.2020.04.019
通讯作者	Yang, Ke(kyang@imr.ac.cn) ; Li, Xiaokang(lxkfmmu@163.com) ; Guo, Zheng(guozheng@fmmu.edu.cn)
英文摘要	The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application. Early angiogenesis is important for scaffold survival. It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties. In this study, a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold. For osseointegration and osteogenesis analysis, in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation, adhesion, extracellular matrix (ECM) mineralization and ALP activity compared with bare Ti6Al4V cocultivation. Additionally, MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesis-related genes expression. In vivo studies including fluorochrome labeling, micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation. For angiogenesis studies, magnesium-coated Ti6Al4V improve HUVECs proliferation, adhesion, tube formation, wound-healing and Transwell abilities. HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes (HIF-1 alpha and VEGF) expression. Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation. This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications.
资助项目	National Key Research and Development Program of China[2017YFC1104901] ; National Natural Science Foundation of China[51871239] ; National Natural Science Foundation of China[51771227] ; National Natural Science Foundation of Youth Fund[51501223]
WOS研究方向	Engineering ; Materials Science
语种	英语
出版者	KEAI PUBLISHING LTD
WOS记录号	WOS:000546576100023
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Youth Fund
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/139586]
专题	金属研究所_中国科学院金属研究所
通讯作者	Yang, Ke; Li, Xiaokang; Guo, Zheng
作者单位	1.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp, Dept Orthoped, Xian 710032, Peoples R China 3.Hunan Normal Univ, Dept Joint Surg & Sports Med, Hunan Prov Peoples Hosp, Changsha 410016, Peoples R China 4.Hunan Normal Univ, Affiliated Hosp 1, Changsha 410016, Peoples R China 5.Shenyang Ligong Univ, Sch Mat Sci & Engn, Shenyang 110159, Peoples R China
推荐引用方式 GB/T 7714	Gao, Peng,Fan, Bo,Yu, Xiaoming,et al. Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application [J]. BIOACTIVE MATERIALS,2020,5(3):680-693.
APA	Gao, Peng.,Fan, Bo.,Yu, Xiaoming.,Liu, Wenwen.,Wu, Jie.,...&Guo, Zheng.(2020).Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application .BIOACTIVE MATERIALS,5(3),680-693.
MLA	Gao, Peng,et al."Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application ".BIOACTIVE MATERIALS 5.3(2020):680-693.