Pulmonary inflammation induced by low-dose particulate matter exposure in mice

doi:10.1152/ajplung.00232.2019

CORC > 地球环境研究所 > 中国科学院地球环境研究所 > 粉尘与环境研究室

	Pulmonary inflammation induced by low-dose particulate matter exposure in mice
	Chan, Yik Lung 7,8; Wang, Baoming 7,8; Chen, Hui 8; Ho, Kin Fai 9; Cao, Junji 1; Hai, Guo 2; Jalaludin, Bin 3; Herbert, Cristan 4,5; Thomas, Paul S.4,5; Saad, Sonia 6
刊名	AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
	2019-09-01
卷号	317 期号:3 页码:L424-L430
ISSN号	1040-0605
DOI	10.1152/ajplung.00232.2019
通讯作者	Chan, Yik Lung(Yik.chan@uts.edu.au)
英文摘要	Air pollution is a ubiquitous problem and comprises gaseous and particulate matter (PM). Epidemiological studies have clearly shown that exposure to PM is associated with impaired lung function and the development of lung diseases, such as chronic obstructive pulmonary disease and asthma. To understand the mechanisms involved, animal models are often used. However, the majority of such models represent high levels of exposure and are not representative of the exposure levels in less polluted countries, such as Australia. Therefore, in this study, we aimed to determine whether low dose PM10 exposure has any detrimental effect on the lungs. Mice were intranasally exposed to saline or traffic-related PM10 (1 mu g or 5 mu g/day) for 3 wk. Bronchoalveolar lavage (BAL) and lung tissue were analyzed. PM10 at 1 mu g did not significantly affect inflammatory and mitochondrial markers. At 5 mu g, PM10 exposure increased lymphocytes and macrophages in BAL fluid. Increased NACHT, LRR and PYD domains-containing protein 3 (NLRP3) and IL-1 beta production occurred following PM10 exposure. PM10 (5 mu g) exposure reduced mitochondrial antioxidant manganese superoxide (antioxidant defense system) and mitochondrial fusion marker (OPA-1), while it increased fission marker (Drp-1). Autophagy marker light-chain 3 microtubule-associated protein (LC3)-II and phosphorylated-AMPK were reduced, and apoptosis marker (caspase 3) was increased. No significant change of remodeling markers was observed. In conclusion, a subchronic low-level exposure to PM can have an adverse effect on lung health, which should be taken into consideration for the planning of roads and residential buildings.
资助项目	SPHERE, RCG of the Hong Kong Special Administrative Region[CRF/C5004-15E] ; Strategic Focus Area scheme of The Research Institute for Sustainable Urban Development at The Hong Kong Polytechnic University[1-BBW9] ; National Natural Science Foundation of China[81750110554] ; National Health and Medical Research Council Australia[APP1110368] ; China Scholarship Council
WOS关键词	NLRP3 INFLAMMASOME ; OXIDATIVE STRESS ; AUTOPHAGY ; PARTICLES ; RESPONSES
WOS研究方向	Physiology ; Respiratory System
语种	英语
出版者	AMER PHYSIOLOGICAL SOC
WOS记录号	WOS:000485282700011
资助机构	SPHERE, RCG of the Hong Kong Special Administrative Region ; Strategic Focus Area scheme of The Research Institute for Sustainable Urban Development at The Hong Kong Polytechnic University ; National Natural Science Foundation of China ; National Health and Medical Research Council Australia ; China Scholarship Council
内容类型	期刊论文
源URL	[http://ir.ieecas.cn/handle/361006/13475]
专题	地球环境研究所_粉尘与环境研究室
通讯作者	Chan, Yik Lung
作者单位	1.Chinese Acad Sci, Inst Earth Environm, Key Lab Aerosol Chem & Phys, Xian, Shaanxi, Peoples R China 2.Hong Kong Polytech Univ, Dept Civil & Environm Engn, Air Qual Studies, Hong Kong, Peoples R China 3.Univ New South Wales, Ingham Inst Appl Med Res, Sydney, NSW, Australia 4.Univ New South Wales, Fac Med, Dept Pathol, Sch Med Sci, Sydney, NSW, Australia 5.Univ New South Wales, Fac Med, Prince Wales Clin Sch, Sydney, NSW, Australia 6.Royal North Shore Hosp, Renal Grp Kolling Inst, St Leonards, NSW, Australia 7.Woolcock Inst Med Res, Resp Cellular & Mol Biol, Sydney, NSW, Australia 8.Univ Technol Sydney, Sch Life Sci, Sydney, NSW, Australia 9.Chinese Univ Hong Kong, Jockey Club Sch Publ Hlth & Primary, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Chan, Yik Lung,Wang, Baoming,Chen, Hui,et al. Pulmonary inflammation induced by low-dose particulate matter exposure in mice[J]. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY,2019,317(3):L424-L430.
APA	Chan, Yik Lung.,Wang, Baoming.,Chen, Hui.,Ho, Kin Fai.,Cao, Junji.,...&Oliver, Brian Gregory George.(2019).Pulmonary inflammation induced by low-dose particulate matter exposure in mice.AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY,317(3),L424-L430.
MLA	Chan, Yik Lung,et al."Pulmonary inflammation induced by low-dose particulate matter exposure in mice".AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY 317.3(2019):L424-L430.