An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates

doi:10.1016/j.chroma.2019.02.064

	An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates
	Wang, Li 1,4; Longo, William M.1; Dillon, James T.1; Zhao, Jiaju 2; Zheng, Yinsui 1; Moros, Matthias 3; Huang, Yongsong 1
刊名	JOURNAL OF CHROMATOGRAPHY A
	2019-07-05
卷号	1596 页码:175-182
关键词	Alkenones Steryl ethers Clean-up Silver thiolate chromatographic material Paleotemperature reconstructions
ISSN号	0021-9673
DOI	10.1016/j.chroma.2019.02.064
通讯作者	Huang, Yongsong(yongsong_huang@brown.edu)
英文摘要	Long-chain alkenones (LCAs) and alkenoates (LCEs) are highly valuable biomarkers for paleotemperature reconstructions. A major problem, however, for accurate quantification of these compounds using gas chromatography (GC) is co-elution with steryl ethers, wax esters, saturated ketones and other numerous mid-polarity compounds frequently encountered in marginal marine and lake sediments. Co-elution during GC separation is prevalent, particularly if the full homologous series of alkenones and alkenoates are to be analyzed. Taking advantage of the presence of two or more double bonds in LCAs and LCEs, the conventional silica gel impregnated with silver nitrate has previously been used to remove co-eluting compounds for LCAs. However, this conventional argentation chromatography is hampered by the extreme instability of silver nitrate, poor reproducibility, low recovery and short lifetime. Here we demonstrate a highly efficient flash chromatographic approach based on silver thiolate chromatographic material (AgTCM) that overcomes the shortcomings of the traditional argentation chromatography and allows repeated sample preparation (up to 62 samples in one test) with little loss in separation efficiency. AgTCM selectively extracts LCAs and LCEs and effectively eliminates co-eluting compounds including steryl ethers and wax esters for the subsequent gas chromatography (GC) analysis. This new method, therefore, allows low-cost and high-throughput sample preparation for comprehensive quantification of the full homologous series of LCAs and LCEs in marine and lake sediments. (C) 2019 Elsevier B.V. All rights reserved.
资助项目	National Science Foundation[EAR-1122749] ; National Science Foundation[PLR-1503846] ; National Science Foundation[EAR-1502455] ; National Science Foundation[EAR-1762431] ; National Science Foundation of China[41502140] ; National Science Foundation of China[XDA14010103]
WOS关键词	LONG-CHAIN ALKENONES ; POLYUNSATURATED FATTY-ACIDS ; NORDIC SEAS ; SEPARATION ; TEMPERATURE ; SEDIMENTS ; SURFACE ; PALEOTEMPERATURE ; IDENTIFICATION ; DISTRIBUTIONS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000469151700019
资助机构	National Science Foundation ; National Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ieecas.cn/handle/361006/13849]
专题	地球环境研究所_古环境研究室
通讯作者	Huang, Yongsong
作者单位	1.Brown Univ, Dept Earth Environm & Planetary Sci, Providence, RI 02912 USA 2.Chinese Acad Sci, Inst Earth Environm, Xian 710071, Peoples R China 3.Leibniz Inst Balt Sea Res, Dept Marine Geol, D-18119 Rostock, Germany 4.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Li,Longo, William M.,Dillon, James T.,et al. An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates[J]. JOURNAL OF CHROMATOGRAPHY A,2019,1596:175-182.
APA	Wang, Li.,Longo, William M..,Dillon, James T..,Zhao, Jiaju.,Zheng, Yinsui.,...&Huang, Yongsong.(2019).An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates.JOURNAL OF CHROMATOGRAPHY A,1596,175-182.
MLA	Wang, Li,et al."An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates".JOURNAL OF CHROMATOGRAPHY A 1596(2019):175-182.