Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review

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	Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review
	Waseem, Hassan ; Jameel, Sana ; Ali, Jafar ; Rehman, Hamza Saleem Ur ; Tauseef, Isfahan ; Farooq, Uzma ; Jamal, Asif ; Ali, Muhammad Ishtiaq
刊名	MOLECULES
	2019
卷号	24 期号:1 页码:-
关键词	AMR high throughput qPCR ARGs MGEs gut microbiome
ISSN号	1420-3049
英文摘要	Expansion in whole genome sequencing and subsequent increase in antibiotic resistance targets have paved the way of high throughput qPCR (HT-qPCR) for analyzing hundreds of antimicrobial resistance genes (ARGs) in a single run. A meta-analysis of 51 selected studies is performed to evaluate ARGs abundance trends over the last 7 years. WaferGen(TM) SmartChip is found to be the most widely used HT-qPCR platform among others for evaluating ARGs. Up till now around 1000 environmental samples (excluding biological replicates) from different parts of the world have been analyzed on HT-qPCR. Calculated detection frequency and normalized ARGs abundance (ARGs/16S rRNA gene) reported in gut microbiome studies have shown a trend of low ARGs as compared to other environmental matrices. Disparities in the HT-qPCR data analysis which are causing difficulties to researchers in precise interpretation of results have been highlighted and a possible way forward for resolving them is also suggested. The potential of other amplification technologies and point of care or field deployable devices for analyzing ARGs have also been discussed in the review. Our review has focused on updated information regarding the role, current status and future perspectives of HT-qPCR in the field of antimicrobial resistance.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43184]
专题	生态环境研究中心_环境纳米材料实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Nanotechnol & Hlth Effects, 18 Shuangqing Rd, Beijing 100085, Peoples R China 2.Michigan State Univ, Dept Environm Engn, E Lansing, MI 48823 USA 3.Univ Sialkot, Dept Biotechnol, Punjab 51310, Pakistan 4.Quaid I Azam Univ, Environm Microbiol Lab, Dept Microbiol, Islamabad 45320, Pakistan 5.Univ Hazara, Dept Microbiol, Mansehra 21300, Pakistan
推荐引用方式 GB/T 7714	Waseem, Hassan,Jameel, Sana,Ali, Jafar,et al. Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review[J]. MOLECULES,2019,24(1):-.
APA	Waseem, Hassan.,Jameel, Sana.,Ali, Jafar.,Rehman, Hamza Saleem Ur.,Tauseef, Isfahan.,...&Ali, Muhammad Ishtiaq.(2019).Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review.MOLECULES,24(1),-.
MLA	Waseem, Hassan,et al."Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review".MOLECULES 24.1(2019):-.