Effects of Arsenic on Gut Microbiota and Its Biotransformation Genes in Earthworm Metaphire sieboldi

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	Effects of Arsenic on Gut Microbiota and Its Biotransformation Genes in Earthworm Metaphire sieboldi
	Wang, Hong-Tao ; Zhu, Dong ; Li, Gang ; Zheng, Fei ; Ding, Jing ; O'Connor, Patrick J.; Zhu, Yong-Guan ; Xue, Xi-Mei
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2019-04-02
卷号	53 期号:7 页码:3841-3849
ISSN号	0013-936X
英文摘要	Arsenic biotransformation mediated by gut microbiota can affect arsenic bioavailability and microbial community. Arsenic species, arsenic biotransformation genes (ABGs), and the composition of gut microbial community were characterized after the earthworm Metaphire sieboldi was cultured in soils spiked with different arsenic concentrations. Arsenite (As(III)) was the major component in the earthworm gut, whereas arsenate (As(V)) was predominant in the soil. A total of 16 ABGs were quantified by high-throughput quantitative polymerase chain reaction (HT-qPCR). Genes involved in arsenic redox and efflux were predominant in all samples, and the abundance of ABGs involved in arsenic methylation and demethylation in the gut was very low. These results reveal that the earthworm gut can be a reservoir of microbes with the capability of reducing As(V) and extruding As(III) but with little methylation of arsenic. Moreover, gut microbial communities were dominated by Actinobacteria, Firmicutes, and Proteobacteria at the phylum level and were considerably different from those in the surrounding soil. Our work demonstrates that exposure to As(V) disturbs the gut microbiota of earthworms and provides some insights into arsenic biotransformation in the earthworm gut.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42720]
专题	生态环境研究中心_城市与区域生态国家重点实验室
作者单位	1.Univ Adelaide, Ctr Global Food & Resources, Adelaide, SA 5005, Australia 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, 1799 Jimei Rd, Xiamen 361021, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hong-Tao,Zhu, Dong,Li, Gang,et al. Effects of Arsenic on Gut Microbiota and Its Biotransformation Genes in Earthworm Metaphire sieboldi[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2019,53(7):3841-3849.
APA	Wang, Hong-Tao.,Zhu, Dong.,Li, Gang.,Zheng, Fei.,Ding, Jing.,...&Xue, Xi-Mei.(2019).Effects of Arsenic on Gut Microbiota and Its Biotransformation Genes in Earthworm Metaphire sieboldi.ENVIRONMENTAL SCIENCE & TECHNOLOGY,53(7),3841-3849.
MLA	Wang, Hong-Tao,et al."Effects of Arsenic on Gut Microbiota and Its Biotransformation Genes in Earthworm Metaphire sieboldi".ENVIRONMENTAL SCIENCE & TECHNOLOGY 53.7(2019):3841-3849.