Heavy metal-induced co-selection of antibiotic resistance genes in the gut microbiota of collembolans

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	Heavy metal-induced co-selection of antibiotic resistance genes in the gut microbiota of collembolans
	Ding, Jing ; An, Xin Li 1; Lassen, Simon Bo 1,2; Wang, Hong Tao 1; Zhu, Dong 1; Ke, Xin 3
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2019-09-15
卷号	683 页码:210-215
关键词	Co-selection ARGs Soil fauna Gut microbiota Synergistic effects
ISSN号	0048-9697
英文摘要	Heavy metal induced co-selection of antibiotic resistance genes (ARGs) has become an emerging environmental issue. The guts of soil fauna offer a unique habitat in the terrestrial ecosystem and harbor a variety of microorganisms. However, the effects of heavy metals on the gut-associated ARGs of soil fauna are poorly understood. In the present study, collembolans were cultivated with four types of heavy metals (Zn, Cu, Cd, and Cr) and one antibiotic (oxytetracycline), to investigate their impact on the gut-associated ARGs. High-throughput quantitative PCR and 16S rRNA gene amplicon sequencing were used to examine changes in the gut-associated ARGs and microbial composition caused by the metals and antibiotic. The results showed that heavy metals alone induced co-selection of ARGs in the collembolan gut, but the effects were weaker than selection by oxytetracycline. When Zn or Cu was present together with oxytetracycline, there was a strong synergistic effect between the compounds, which increased the selection of ARGs in the collembolan guts. Furthermore, redundancy analysis revealed that the gut microbiota and mobile genetic elements (MGEs) were significantly correlated with the ARG composition. These results extend our understanding on effects of heavy metals on the dispersal of ARGs in the soil food web. (C) 2019 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42422]
专题	生态环境研究中心_城市与区域生态国家重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, 18 Shuangqing Rd, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, 1799 Jimei Rd, Xiamen 361021, Fujian, Peoples R China 3.Univ Copenhagen, Fac Sci, Dept Plant & Environm Sci, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Ding, Jing,An, Xin Li,Lassen, Simon Bo,et al. Heavy metal-induced co-selection of antibiotic resistance genes in the gut microbiota of collembolans[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,683:210-215.
APA	Ding, Jing,An, Xin Li,Lassen, Simon Bo,Wang, Hong Tao,Zhu, Dong,&Ke, Xin.(2019).Heavy metal-induced co-selection of antibiotic resistance genes in the gut microbiota of collembolans.SCIENCE OF THE TOTAL ENVIRONMENT,683,210-215.
MLA	Ding, Jing,et al."Heavy metal-induced co-selection of antibiotic resistance genes in the gut microbiota of collembolans".SCIENCE OF THE TOTAL ENVIRONMENT 683(2019):210-215.