Targeted live-cell nuclear delivery of the DNA 'light-switching' Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Targeted live-cell nuclear delivery of the DNA 'light-switching' Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes
	Chao, Xi-Juan ; Tang, Miao ; Huang, Rong ; Huang, Chun-Hua ; Shao, Jie ; Yan, Zhu-Ying ; Zhu, Ben-Zhan
刊名	NUCLEIC ACIDS RESEARCH
	2019-11-18
卷号	47 期号:20 页码:10520-10528
ISSN号	0305-1048
英文摘要	We have found recently that nuclear uptake of the cell-impermeable DNA light-switching Ru(II)-polypyridyl cationic complexes such as [Ru(bpy)(2)(dppz)]Cl-2 was remarkably enhanced by pentachlorophenol (PCP), by forming ion-pairing complexes via a passive diffusion mechanism. However, it is not clear whether the enhanced nuclear uptake of [Ru(bpy)(2)(dppz)](2+) is only limited to PCP, or it is a general phenomenon for other highly chlorinated phenols (HCPs); and if so, what are the major physicochemical factors in determining nuclear uptake? Here, we found that the nuclear uptake of [Ru(bpy)(2)(dppz)](2+) can also be facilitated by other two groups of HCPs including three tetrachlorophenol (TeCP) and six trichlorophenol (TCP) isomers. Interestingly and unexpectedly, 2,3,4,5-TeCP was found to be the most effective one for nuclear delivery of [Ru(bpy)(2)(dppz)](2+), which is even better than the most-highly chlorinated PCP, and much better than its two other TeCP isomers. Further studies showed that the nuclear uptake of [Ru(bpy)(2)(dppz)](2+) was positively correlated with the binding stability, but to our surprise, inversely correlated with the lipophilicity of the ion-pairing complexes formed between [Ru(bpy)(2)(dppz)]Cl-2 and HCPs. These findings should provide new perspectives for future investigations on using ion-pairing as an effective method for delivering other bio-active metal complexes into their intended cellular targets.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42602]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Yan, Zhu-Ying 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 3.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100085, Peoples R China 4.Sun Yat Sen Univ, Sch Chem, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Guangdong, Peoples R China 5.Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA 6.Res Ctr Ecoenvironm Sci, Joint Inst Environm Sci, Hong Kong, Peoples R China 7.Hong Kong Baptist Univ, Hong Kong, Peoples R China 8.Shao, Jie 9.Huang, Chun-Hua 10.Huang, Rong
推荐引用方式 GB/T 7714	Chao, Xi-Juan,Tang, Miao,Huang, Rong,et al. Targeted live-cell nuclear delivery of the DNA 'light-switching' Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes[J]. NUCLEIC ACIDS RESEARCH,2019,47(20):10520-10528.
APA	Chao, Xi-Juan.,Tang, Miao.,Huang, Rong.,Huang, Chun-Hua.,Shao, Jie.,...&Zhu, Ben-Zhan.(2019).Targeted live-cell nuclear delivery of the DNA 'light-switching' Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes.NUCLEIC ACIDS RESEARCH,47(20),10520-10528.
MLA	Chao, Xi-Juan,et al."Targeted live-cell nuclear delivery of the DNA 'light-switching' Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes".NUCLEIC ACIDS RESEARCH 47.20(2019):10520-10528.