Intracellular Dissolution of Silver Nanoparticles: Evidence from Double Stable Isotope Tracing

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Intracellular Dissolution of Silver Nanoparticles: Evidence from Double Stable Isotope Tracing
	Yu, Su-juan ; Lai, Yu-jian ; Dong, Li-jie ; Lie, Jing-fu
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2019-09-03
卷号	53 期号:17 页码:10218-10226
ISSN号	0013-936X
英文摘要	To track transformations of silver nanoparticles (AgNPs) in vivo, HepG2 and AS49 cells were cocultured with two enriched stable Ag isotopes ((107)AgNPs and (AgNO3)-Ag-109) at nontoxic doses. After enzymatic digestion, (107)AgNPs, ionic Ag-107(+) and Ag-109(+) in exposed cells could be separated and quantified by liquid chromatography combined with ICP-MS. We found that ratios of Ag-107(+) to total Ag-107 and proportions of Ag-107(+)/in cells increased gradually after exposure, proving that the Trojan-horse mechanism occurred, i.e., AgNPs released high contents of Ag+ after internalization. While the presence of Ag-109(+) (5 and 100 mu g/L) has little influence on the uptake of (107)AgNPs (0.1 and 2 mg/L), the presence of (107)AgNPs at a high dose (2 mg/L) dramatically increases the ingestion of Ag-109(+), even though (107)AgNPs at a low dose (100 mu g/L) showed negligible effects on the internalization of Ag-109(+). Cellular homeostasis may be perturbed under sublethal exposure of (107)AgNPs, and thus enhanced uptake of Ag-109(+). Our findings suggest that the widely adopted control experiments in toxicology studies, culturing organisms with AgNO3 at the same concentration of Ag+ in the AgNP exposure medium, may underestimate uptake of Ag+ and thus cannot exclude suspected toxic effects of Ag+ at high AgNP exposure doses.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42552]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, POB 2871, Beijing 100085, Peoples R China 2.[Yu, Su-juan 3.Lai, Yu-jian 4.Dong, Li-jie
推荐引用方式 GB/T 7714	Yu, Su-juan,Lai, Yu-jian,Dong, Li-jie,et al. Intracellular Dissolution of Silver Nanoparticles: Evidence from Double Stable Isotope Tracing[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2019,53(17):10218-10226.
APA	Yu, Su-juan,Lai, Yu-jian,Dong, Li-jie,&Lie, Jing-fu.(2019).Intracellular Dissolution of Silver Nanoparticles: Evidence from Double Stable Isotope Tracing.ENVIRONMENTAL SCIENCE & TECHNOLOGY,53(17),10218-10226.
MLA	Yu, Su-juan,et al."Intracellular Dissolution of Silver Nanoparticles: Evidence from Double Stable Isotope Tracing".ENVIRONMENTAL SCIENCE & TECHNOLOGY 53.17(2019):10218-10226.