Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms

doi:10.3389/fmicb.2019.02863

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	Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms
	Zhang, Yimeng 2,3,4,5; Ma, Yan 2,3,4; Zhang, Ruiyong 1; Zhang, Binbin 2,3,4; Zhai, Xiaofan 2,3,4; Li, Wangqiang 2,3,4; Xu, Liting 2,3,4; Jiang, Quantong 2,3,4; Duan, Jizhou 2,3,4; Hou, Baorong 2,3,4
刊名	FRONTIERS IN MICROBIOLOGY
	2019-12-11
卷号	10 页码:13
关键词	marine biofilm biocorrosion copper-resistance metal alloy gene biofouling
ISSN号	1664-302X
DOI	10.3389/fmicb.2019.02863
通讯作者	Duan, Jizhou(duanjz@qdio.ac.cn)
英文摘要	We used metagenomic sequencing combined with morphological and chemical analyses to investigate microbial taxa and functions related to copper-resistance and microbiologically influenced corrosion in mature copper-associated biofilms in coastal seawater for 44 months. Facultative anaerobic microbes such as Woeseia sp. were found to be the dominant groups on the copper surface. Genes related to stress response and possible heavy metal transport systems, especially RNA polymerase sigma factors (rpoE) and putative ATP-binding cassette (ABC) transport system permease protein (ABC.CD.P) were observed to be highly enriched in copper-associated biofilms, while genes encoding DNA-methyltransferase and RNA polymerase subunit were highly enriched in aluminum-associated biofilms and seawater planktonic cells, respectively. Moreover, copper-associated biofilms harbored abundant copper-resistance genes including cus, cop and pco, as well as abundant genes related to extracellular polymeric substances, indicating the presence of diverse copper-resistance patterns. The proportion of dsr in copper-associated biofilms, key genes related to sulfide production, was as low as that in aluminum biofilm and seawater, which ruled out the possibility of microbial sulfide-induced copper-corrosion under field conditions. These results may fill knowledge gaps about the in situ microbial functions of marine biofilms and their effects on toxic-metal corrosion.
资助项目	National Natural Science Foundation of China[41576080] ; Key Research and Development Plan of Shandong Province[2018GHY115003] ; Key Research and Development Plan of Shandong Province[2018GHY115029] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA13040403] ; Fundamental Research Project of Technology Program of Qingdao[17-1-1-76-JCH]
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000504235400001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/164151]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Duan, Jizhou
作者单位	1.Fed Inst Geosci & Nat Resources, Hannover, Germany 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao, Shandong, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, Qingdao, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yimeng,Ma, Yan,Zhang, Ruiyong,et al. Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms[J]. FRONTIERS IN MICROBIOLOGY,2019,10:13.
APA	Zhang, Yimeng.,Ma, Yan.,Zhang, Ruiyong.,Zhang, Binbin.,Zhai, Xiaofan.,...&Hou, Baorong.(2019).Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms.FRONTIERS IN MICROBIOLOGY,10,13.
MLA	Zhang, Yimeng,et al."Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms".FRONTIERS IN MICROBIOLOGY 10(2019):13.