Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection

doi:10.1002/mbo3.1150

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection
	Huang, Sijun 1; Sun, Yingting 1,2; Zhang, Si 1; Long, Lijuan 1
刊名	MICROBIOLOGYOPEN
	2020-12-30
页码	11
关键词	cyanophage genome cyanopodovirus RNA‐ seq Synechococcus transcriptome
ISSN号	2045-8827
DOI	10.1002/mbo3.1150
通讯作者	Huang, Sijun(huangsijun@scsio.ac.cn) ; Long, Lijuan(longlj@scsio.ac.cn)
英文摘要	Marine picocyanobacteria belonging to genera Synechococcus and Prochlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus-host systems share similar gene expression patterns during infection. Whole-genome expression dynamics of T7-like cyanopodovirus P-SSP7 and its host Prochlorococcus strain MED4 have already been reported. Here, we conducted genomic and transcriptomic analyses on T7-like cyanopodovirus S-SBP1 during its infection on Synechococcus strain WH7803. S-SBP1 has a latent period of 8 h and phage DNA production of 30 copies per cell. In terms of whole-genome phylogenetic relationships and average nucleotide identity, S-SBP1 was most similar to cyanopodovirus S-RIP2, which also infects Synechococcus WH7803. Three hypervariable genomic islands were identified when comparing the genomes of S-SBP1 and S-RIP2. Single nucleotide variants were also observed in three S-SBP1 genes, which were located within the island regions. Based on RNA-seq analysis, S-SBP1 genes clustered into three temporal expression classes, whose gene content was similar to that of P-SSP7. Thirty-two host genes were upregulated during phage infection, including those involved in carbon metabolism, ribosome components, and stress response. These upregulated genes were similar to those upregulated by Prochlorococcus MED4 in response to infection by P-SSP7. Our study demonstrates a programmed temporal expression pattern of cyanopodoviruses and hosts during infection.
资助项目	National Natural Science Foundation of China[41576126] ; Natural Science Foundation of Guangdong Province[2017A030306020] ; Youth Innovation Promotion Association[2018377] ; Rising Star Foundation of the South China Sea Institute of Oceanology[NHXX2019ST0101] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA13020301]
WOS研究方向	Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000603422100001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/169501]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Huang, Sijun; Long, Lijuan
作者单位	1.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Huang, Sijun,Sun, Yingting,Zhang, Si,et al. Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection[J]. MICROBIOLOGYOPEN,2020:11.
APA	Huang, Sijun,Sun, Yingting,Zhang, Si,&Long, Lijuan.(2020).Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection.MICROBIOLOGYOPEN,11.
MLA	Huang, Sijun,et al."Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection".MICROBIOLOGYOPEN (2020):11.