Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons

doi:10.1007/s00343-020-0040-7

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons
	Li Mengna 1,2,5; Chen Hao 1,2,3,4; Wang Minxiao 1,2,3,4; Zhong Zhaoshan 1,2,5; Zhou Li 1,2,3,4; Li Chaolun 1,2,3,4,5
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2020-07-01
卷号	38 期号:4 页码:1292-1303
关键词	Gigantidas platifrons endosymbiosis innate immunity pull-down assay immune recognition methane oxidation bacteria
ISSN号	2096-5508
DOI	10.1007/s00343-020-0040-7
通讯作者	Li Chaolun(lcl@qdio.ac.cn)
英文摘要	Deep-sea mussels of the subfamily Bathymodiolinae are common and numerically dominant species widely distributed in cold seeps and hydrothermal vents. During long-time evolution, deep-sea mussels have evolved to be well adapted to the local environment of cold seeps and hydrothermal vents by various ways, especially by establishing endosymbiosis with chemotrophic bacteria. However, biological processes underlying the establishment and maintenance of symbiosis between host mussels and symbionts are largely unclear. In the present study,Gigantidas platifronsgenes possibly involved in the symbiosis with methane oxidation symbionts were identified and characterized by Lipopolysaccharide (LPS) pull-down andin situhybridization. Five immune related proteins including Toll-like receptor 2 (TLR2), integrin, vacuolar sorting protein (VSP), matrix metalloproteinase 1 (MMP1), and leucine-rich repeat (LRR-1) were identified by LPS pull-down assay. These five proteins were all conserved in either molecular sequences or functional domains and known to be key molecules in host immune recognition, phagocytosis, and lysosome-mediated digestion. Furthermore,in situhybridization of LRR-1, TLR2 and VSP genes was conducted to investigate their expression patterns in gill tissues ofG. platifrons. Consequently, LRR-1, TLR2, and VSP genes were found expressed exclusively in the bacteriocytes ofG. platifrons. Therefore, it was suggested that TLR2, integrin, VSP, MMP1, and LRR-1 might be crucial molecules in the symbiosis betweenG. platifronsand methane oxidation bacteria by participating in symbiosis-related immune processes.
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000551529700026
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168041]
专题	海洋研究所_海洋生态与环境科学重点实验室海洋研究所_深海极端环境与生命过程研究中心
通讯作者	Li Chaolun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci CODR & KLMEES, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li Mengna,Chen Hao,Wang Minxiao,et al. Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(4):1292-1303.
APA	Li Mengna,Chen Hao,Wang Minxiao,Zhong Zhaoshan,Zhou Li,&Li Chaolun.(2020).Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(4),1292-1303.
MLA	Li Mengna,et al."Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.4(2020):1292-1303.