A C-Type Lectin Highly Expressed in Portunus trituberculatus Intestine Functions in AMP Regulation and Prophenoloxidase Activation

doi:10.3390/antibiotics10050541

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	A C-Type Lectin Highly Expressed in Portunus trituberculatus Intestine Functions in AMP Regulation and Prophenoloxidase Activation
	Liu, Yuan 1,3,4,5,6; Su, Yue 3,5,6; Zhang, Ao 5,6; Cui, Zhaoxia 2
刊名	ANTIBIOTICS-BASEL
	2021-05-01
卷号	10 期号:5 页码:23
关键词	C-type lectin Portunus trituberculatus innate immune recognition AMP expression proPO system
ISSN号	2079-6382
DOI	10.3390/antibiotics10050541
通讯作者	Liu, Yuan(liuyuan@qdio.ac.cn)
英文摘要	A C-type lectin (PtCLec2) from Portunus trituberculatus was identified for characterization of its role in defense and innate immunity. PtCLec2 contains a single carbohydrate-recognition domain (CRD) with a conserved QPD motif, which was predicted to have galactose specificity. The mRNA expression of PtCLec2 was predominantly detected in intestine and increased rapidly and significantly upon pathogen challenge. The recombinant PtCLec2 (rPtCLec2) could bind various microorganisms and PAMPs with weak binding ability to yeast and PGN. It agglutinated the tested Gram-negative bacteria (Vibrio alginolyticus and Pseudomonas aeruginosa), Gram-positive bacteria (Staphylococcus aureus and Micrococcus luteus), and rabbit erythrocytes in the presence of exogenous Ca2+, and these agglutination activities were suppressed by LPS, d-galactose, and d-mannose. Further, rPtCLec2 enhanced phagocytosis and clearance of V. alginolyticus, and displayed inhibitory activities against the tested bacteria. Knockdown of PtCLec2 decreased the transcription of two phagocytosis genes (PtArp and PtMyosin), three prophenoloxidase (proPO) system-related genes (PtPPAF, PtcSP1, and PtproPO), six antimicrobial peptides (AMPs) (PtALF4-7, PtCrustin1, and PtCrustin3), and PtRelish but upregulated the expression levels of PtJNK, PtPelle, and PtTLR. These results collectively indicate that PtCLec2 might perform its immune recognition function via binding and agglutination, and mediate pathogen elimination via regulating hemocyte phagocytosis, AMP synthesis, and proPO activation.
资助项目	National Key R&D Program of China[2018YFD0901304] ; National Natural Science Foundation of China[41776159] ; Applied Basic Research Program of Science and Technology Department of Qingdao[17-1-1-94-jch] ; China Agriculture Research System-48 (CARS-48)
WOS研究方向	Infectious Diseases ; Pharmacology & Pharmacy
语种	英语
出版者	MDPI
WOS记录号	WOS:000653374500001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170889]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Yuan
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Qingdao 266071, Peoples R China 6.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuan,Su, Yue,Zhang, Ao,et al. A C-Type Lectin Highly Expressed in Portunus trituberculatus Intestine Functions in AMP Regulation and Prophenoloxidase Activation[J]. ANTIBIOTICS-BASEL,2021,10(5):23.
APA	Liu, Yuan,Su, Yue,Zhang, Ao,&Cui, Zhaoxia.(2021).A C-Type Lectin Highly Expressed in Portunus trituberculatus Intestine Functions in AMP Regulation and Prophenoloxidase Activation.ANTIBIOTICS-BASEL,10(5),23.
MLA	Liu, Yuan,et al."A C-Type Lectin Highly Expressed in Portunus trituberculatus Intestine Functions in AMP Regulation and Prophenoloxidase Activation".ANTIBIOTICS-BASEL 10.5(2021):23.