Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus

doi:10.1007/s11033-021-06342-9

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus
	Wang, Ling 1,2,3,5; Tan, Xungang 1,2,5; Zou, Congcong 1,2,3,5; Wang, Lijuan 1,2,5; Wu, Zhihao 1,2,5; Zou, Yuxia 1,2,5; Song, Zongcheng 4; You, Feng 1,2,5
刊名	MOLECULAR BIOLOGY REPORTS
	2021-04-20
页码	12
关键词	Dnali1 Repeated fragment Sexually dimorphic expression Gonad 5′ UTR 3′ UTR
ISSN号	0301-4851
DOI	10.1007/s11033-021-06342-9
通讯作者	Tan, Xungang(tanx@qdio.ac.cn) ; You, Feng(youfeng@qdio.ac.cn)
英文摘要	Dynein axonemal light intermediate chain 1 (dnali1) is an important part of axonemal dyneins and plays an important role in the growth and development of animals. However, there is little information about dnali1 in fish. Herein, we cloned dnali1 gene from the genome of olive flounder (Paralichthys olivaceus), a commercially important maricultured fish in China, Japan, and Korea, and analyzed its expression patterns in different gender fish. The flounder dnali1 DNA sequence contained a 771 bp open reading frame (ORF), two different sizes of 5 ' untranslated region (5 ' UTR), and a 1499 bp 3 ' untranslated region (3 ' UTR). Two duplicated 922 nt fragments were found in dnali1 mRNA. The first fragment contained the downstream coding region and the front portion of 3 ' UTR, and the second fragment was entirely located in 3 ' UTR. Multiple alignments indicated that the flounder Dnali1 protein contained the putative conserved coiled-coil domain. Its expression showed sexually dimorphic with predominant expression in the flounder testis, and lower expression in other tissues. The gene with the longer 5 ' UTR was specifically expressed in the testis. The highest expression level in the testis was detected at stages IV and V. Transient expression analysis showed that the 922 bp repeated sequence 3 ' UTR of dnali1 down-regulated the expression of GFP at the early stage in zebrafish. The flounder dnali1 might play an important role in the testis, especially in the period of spermatogenesis, and the 5 ' UTR and the repetitive sequences in 3 ' UTR might contain some regulatory elements for the cilia.
资助项目	National Sciences of Foundation of China[31772834] ; National Sciences of Foundation of China[31672636] ; National Sciences of Foundation of China[31702337] ; National Key R&D Program of China[2018YFD0900202] ; Key Technology Research and Development Program of Shandong (CN)[2018YFD0901202]
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000641648600003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170624]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Tan, Xungang; You, Feng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 4.Shenghang Aquat Sci & Technol Co Ltd, Weihai, Peoples R China 5.Chinese Acad Sci, CAS, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ling,Tan, Xungang,Zou, Congcong,et al. Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus[J]. MOLECULAR BIOLOGY REPORTS,2021:12.
APA	Wang, Ling.,Tan, Xungang.,Zou, Congcong.,Wang, Lijuan.,Wu, Zhihao.,...&You, Feng.(2021).Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus.MOLECULAR BIOLOGY REPORTS,12.
MLA	Wang, Ling,et al."Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus".MOLECULAR BIOLOGY REPORTS (2021):12.