Cadmium sulfide nanoparticle biomineralization and biofilm formation mediate cadmium resistance of the deep-sea bacterium Pseudoalteromonas sp. MT33b

doi:10.1111/1758-2229.12933

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Cadmium sulfide nanoparticle biomineralization and biofilm formation mediate cadmium resistance of the deep-sea bacterium Pseudoalteromonas sp. MT33b
	Ma, Ning 1,2,3,4,5; Sun, Chaomin 1,3,4,5
刊名	ENVIRONMENTAL MICROBIOLOGY REPORTS
	2021-02-07
页码	12
ISSN号	1758-2229
DOI	10.1111/1758-2229.12933
通讯作者	Sun, Chaomin(sunchaomin@qdio.ac.cn)
英文摘要	Cadmium (Cd) is a common toxic heavy metal in the environment, and bacteria have evolved different strategies to deal with Cd toxicity. Here, a bacterium designated Pseudoalteromonas sp. MT33b possessing strong Cd resistance was isolated from the Mariana Trench sediments. Supplement of cysteine significantly increased bacterial Cd resistance and removal rate. Biofilm formation was demonstrated to play a positive role toward bacterial Cd resistance. Transcriptome analysis showed the supplement of cysteine effectively prevented Cd2+ from entering bacterial cells, promoted saccharide metabolism and thereby facilitating energy production, which consists well with bacterial growth trend analysed under the same conditions. Notably, the expressions of many biofilm formation related genes including flagellar assembly, signal transduction, bacterial secretion and TonB-dependent transfer system were significantly upregulated when facing Cd stress, indicating their important roles in determining bacterial biofilm formation and enhancing Cd resistance. Overall, this study indicates the formation of insoluble CdS precipitates and massive biofilm is the major strategy adopted by Pseudoalteromonas sp. MT33b to eliminate Cd stress. Our results provide a good model to investigate how heavy metals impact biofilm formation in the deep-sea ecosystems, which may facilitate a deeper understanding of microbial environmental adaptability and better utilization of these microbes for bioremediation purposes in the future.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; Major Research Plan of the National Natural Science Foundation[92051107] ; Key Deployment Projects of Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04] ; National Key R and D Program of China[2018YFC0310800] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Program[18-1-2-7-zhc]
WOS研究方向	Environmental Sciences & Ecology ; Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000615784900001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/169670]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_深海极端环境与生命过程研究中心
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Ma, Ning,Sun, Chaomin. Cadmium sulfide nanoparticle biomineralization and biofilm formation mediate cadmium resistance of the deep-sea bacterium Pseudoalteromonas sp. MT33b[J]. ENVIRONMENTAL MICROBIOLOGY REPORTS,2021:12.
APA	Ma, Ning,&Sun, Chaomin.(2021).Cadmium sulfide nanoparticle biomineralization and biofilm formation mediate cadmium resistance of the deep-sea bacterium Pseudoalteromonas sp. MT33b.ENVIRONMENTAL MICROBIOLOGY REPORTS,12.
MLA	Ma, Ning,et al."Cadmium sulfide nanoparticle biomineralization and biofilm formation mediate cadmium resistance of the deep-sea bacterium Pseudoalteromonas sp. MT33b".ENVIRONMENTAL MICROBIOLOGY REPORTS (2021):12.