Sphingosinithalassobacter tenebrarum sp. nov., isolated from a deep-sea cold seep

doi:10.1099/ijsem.0.004448

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	Sphingosinithalassobacter tenebrarum sp. nov., isolated from a deep-sea cold seep
	Zheng, Rikuan 1,2,3,4; Sun, Chaomin 1,2,4
刊名	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
	2020
卷号	70 期号:10 页码:5561-5566
ISSN号	1466-5026
DOI	10.1099/ijsem.0.004448
通讯作者	Sun, Chaomin(sunchaomin@qdio.ac.cn)
英文摘要	A Gram-stain-negative, facultatively anaerobic, yellow-pigmented, non-motile, rod-shaped bacterium, designated zrk23(T), was isolated from a deep-sea cold seep. The strain was characterized by a polyphasic approach to clarify its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences placed zrk23(T) within the genus Sphingosinithalassobacter and showed the highest similarity to Sphingosinithalassobacter portus FM6(T) (97.93%). Growth occurs at temperatures from 16 to 45 degrees C (optimum, 30 degrees C), at pH values between pH 6.0 and 8.5 (optimum, pH 7.0) and in 0-5.0% (w/v) NaCl (optimum, 1.5%). The major fatty acids were C-16:0, C-14:0 2-OH and summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c). The major isoprenoid quinone was ubiquinone-10. Predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unidentified phosphoglycolipid, three unidentified glycolipids and three unidentified phospholipids. The G+C content of the genomic DNA was 64.69 %. The average nucleotide identity values between zrk23(T) and the most closely related available genome, of Sphingosinithalassobacter portus FM6(T), was 82.21%, indicating that zrk23(T) was clearly distinguished from S. portus. The analysis of genome sequence of zrk23(T) revealed that there were many genes associated with degradation of aromatic compounds existing in the genome of zrk23(T). As a result of the combination of the results of phylogenetic analysis and phenotypic and chemotaxonomic data, zrk23(T) was considered to represent a novel species of the genus Sphingosinithalassobacter, for which the name Sphingosinithalassobacter tenebrarum sp. nov. is proposed. The type strain is zrk23(T) (=KCTC 72896(T)=MCCC 1K04416(T)).
资助项目	National Key R and D Programme of China[2018YFC0310800] ; China Ocean Mineral Resources R and D Association Grant[DY135-B2-14] ; Strategic Priority Research Programme of the Chinese Academy of Sciences[XDA22050301] ; Taishan Young Scholar Programme of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Programme[18-1-2-7-zhc]
WOS研究方向	Microbiology
语种	英语
出版者	MICROBIOLOGY SOC
WOS记录号	WOS:000582680200045
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168983]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Chaomin
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Rikuan,Sun, Chaomin. Sphingosinithalassobacter tenebrarum sp. nov., isolated from a deep-sea cold seep[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2020,70(10):5561-5566.
APA	Zheng, Rikuan,&Sun, Chaomin.(2020).Sphingosinithalassobacter tenebrarum sp. nov., isolated from a deep-sea cold seep.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,70(10),5561-5566.
MLA	Zheng, Rikuan,et al."Sphingosinithalassobacter tenebrarum sp. nov., isolated from a deep-sea cold seep".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 70.10(2020):5561-5566.