Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition

doi:10.1111/1462-2920.15237

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition
	Wu, Zuodong 3; Zheng, Rikuan 1,2,4; Liu, Ge 1,2,4; Liu, Rui 1,2,4; Wu, Shimei 3; Sun, Chaomin 1,2,4
刊名	ENVIRONMENTAL MICROBIOLOGY
	2020-09-29
页码	13
ISSN号	1462-2912
DOI	10.1111/1462-2920.15237
通讯作者	Wu, Shimei(shimeiwu2016@126.com) ; Sun, Chaomin(sunchaomin@qdio.ac.cn)
英文摘要	Cadmium (Cd) is a common toxic heavy metal in the environment, and bacteria have evolved different strategies against Cd-toxicity. Here, we found that marine bacteriumBacillussp. 98 could significantly alleviate Cd-toxicity by recruiting calcium (Ca) for reducing excessive intracellular nitric oxide (NO) and enhancing iron acquisition. To investigate the underlying mechanisms, mass spectrometry-based proteomic analysis was applied toBacillussp. 98 after treated with Cd supplemented with or without Ca. Compared with bacterial cells treated with Cd only, the proteomic results showed that the expression level of NO synthase was markedly down-regulated, while the expression levels of NO dioxygenase, which is responsible for converting NO to nitrate, and proteins associated with iron uptake were profoundly enhanced when Ca was supplemented. Consistently, bacterial intracellular NO amount was dramatically increased afterBacillussp. 98 was treated with Cd, and reversed to a normal level when Ca or iron was supplemented. Notably, Ca also protected bacteria against stresses from other heavy metals including Cu, Cr, Mn, Ni and Zn, and this self-protection strategy was adopted as well in zebrafish, which encourages us to develop Ca-associated products against heavy metals toxicity in the future.
资助项目	China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; National Key R and D Program of China[2018YFC0310800] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Program[18-1-2-7-zhc]
WOS研究方向	Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000573469300001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168858]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wu, Shimei; Sun, Chaomin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 3.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wu, Zuodong,Zheng, Rikuan,Liu, Ge,et al. Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition[J]. ENVIRONMENTAL MICROBIOLOGY,2020:13.
APA	Wu, Zuodong,Zheng, Rikuan,Liu, Ge,Liu, Rui,Wu, Shimei,&Sun, Chaomin.(2020).Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition.ENVIRONMENTAL MICROBIOLOGY,13.
MLA	Wu, Zuodong,et al."Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition".ENVIRONMENTAL MICROBIOLOGY (2020):13.