CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda

doi:10.1016/j.ydbio.2020.07.001

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	CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda
	Gao, Yi 1,2,4; Zhang, Xiaoxi 1,3; Zhang, Xiaojun 1,2,4; Yuan, Jianbo 1,2,4; Xiang, Jianhai 1,2; Li, Fuhua 1,2,4
刊名	DEVELOPMENTAL BIOLOGY
	2020-09-15
卷号	465 期号:2 页码:157-167
关键词	Development Compound eye Pax6 CRISPR/Cas9 Crustaceans
ISSN号	0012-1606
DOI	10.1016/j.ydbio.2020.07.001
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
英文摘要	The compound eye in crustaceans is a main eye type in the animal kingdom, knowledge about the mechanism to determine the development of compound eye is very limited. Paired box protein 6 (Pax6) is generally regarded as a master regulator for eye development. In the present study, a genome-based analysis of the Pax6 gene in the ridge tail white prawn Exopalaemon carinicauda was performed and two members of Pax6 homologs, named Ec-Eyeless (EcEy) and Ec-Twin of eyeless (EcToy) were identified. To understand the function of these two homologs of Pax6 gene in the prawn, the CRISPR/Cas9 genome editing technique was applied to generate EcEy and EcToy knock-out (KO) prawns and their phenotypes were analyzed. The surviving EcEy-KO embryos and larvae exhibited severe abnormal eye morphology, suggesting that EcEy is necessary for the compound eye development in prawn, while no mutant phenotype was found in EcToy-KO individuals. These findings highlighted the conservative role of Pax6 gene in the compound eye formation, and the functional differentiation between EcEy and EcToy gene may reveal a novel regulating mechanism of Pax6 on the compound eye development in the decapods. These data will provide important information for understanding the regulation mechanism for crustacean compound eye development.
资助项目	National Key R&D Program of China[2018YFD0901301] ; National Key R&D Program of China[2018YFD0900404] ; Major Science and Technology Innovation Projects in Shandong Province[2018SDKJ0302-5] ; National Natural Science Foundation of China[31702320]
WOS研究方向	Developmental Biology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000567412300008
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168734]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yi,Zhang, Xiaoxi,Zhang, Xiaojun,et al. CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda[J]. DEVELOPMENTAL BIOLOGY,2020,465(2):157-167.
APA	Gao, Yi,Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,Xiang, Jianhai,&Li, Fuhua.(2020).CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda.DEVELOPMENTAL BIOLOGY,465(2),157-167.
MLA	Gao, Yi,et al."CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda".DEVELOPMENTAL BIOLOGY 465.2(2020):157-167.