Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus

doi:10.1016/j.aquatox.2020.105580

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	Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus
	Zhu, Long 2; Yuan, Cong 4; Wang, Mingrong 2; Liu, Yan 3; Wang, Zaizhao 2; Seif, Mohamed M.1,2
刊名	AQUATIC TOXICOLOGY
	2020-09-01
卷号	226 页码:11
关键词	Bisphenol A DNA methylation Histone methylation Gobiocypris rarus
ISSN号	0166-445X
DOI	10.1016/j.aquatox.2020.105580
通讯作者	Wang, Zaizhao(zzwang@nwsuaf.edu.cn)
英文摘要	Bisphenol A (BPA), a well-known estrogenic endocrine disruptor, is ubiquitously present in the environment, possessing the potential to interfere with the reproductive endocrine system in male mammals. However, there are limited studies on the reproductive toxicity in male aquatic animals associated with epigenetic modifications. In order to evaluate the potential effects of BPA on reproduction and better understand the underlying mechanism, adult male rare minnow (Gobiocypris rarus) were exposed to 15 mu g L-1 BPA over a period of 63 d. Results showed that BPA induced congestion of blood vessels and infiltration of inflammatory cells after 21 d exposure, and decreased sperm fertilization after 63 d exposure. The genome DNA methylation levels were significantly increased throughout the treatment, and a strong positive stain were found in the spermatocyte, spermatid and sperm. The H3K4me3 level in all types of germ cell were increased by 21 d exposure while decreased following 63 d exposure. The positive stain of H3K9me3 was decreased in sperms while increased in spermatids by 21 d exposure. In addition, the H3K9me3 level was significantly increased after 63 d exposure, and a strong positive stain were found in spermatocytes, spermatids, and sperms. Our result also revealed that the transcripts of DNA methyltransferase genes (dnmt1 and dnmt3-8) and histone methyltransferase genes (mll2-5, setdb1-2 and ezh2) were also markedly changed under BPA exposure for 21-63 d. These findings indicated that BPA had toxicity in male reproductive, and DNA/histone methylation might play a vital role in the regulation of BPA-triggered the decreased of sperm quality.
资助项目	National Natural Science Foundation of China[31670523]
WOS研究方向	Marine & Freshwater Biology ; Toxicology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000565498500012
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168477]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Zaizhao
作者单位	1.Natl Res Ctr, Toxicol & Food Contaminants Dept, Cairo 11435, Egypt 2.Northwest A&F Univ, Coll Anim Sci & Technol, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Lanzhou Univ, Sch Life Sci, Gansu Key Lab Biomonitoring & Bioremediat Environ, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Long,Yuan, Cong,Wang, Mingrong,et al. Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus[J]. AQUATIC TOXICOLOGY,2020,226:11.
APA	Zhu, Long,Yuan, Cong,Wang, Mingrong,Liu, Yan,Wang, Zaizhao,&Seif, Mohamed M..(2020).Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus.AQUATIC TOXICOLOGY,226,11.
MLA	Zhu, Long,et al."Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus".AQUATIC TOXICOLOGY 226(2020):11.