The immune function of a novel crustin with an atypical WAP domain in regulating intestinal microbiota homeostasis in Litopenaeus vannamei

doi:10.1016/j.dci.2020.103756

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	The immune function of a novel crustin with an atypical WAP domain in regulating intestinal microbiota homeostasis in Litopenaeus vannamei
	Lv, Xinjia 1,2,3,5; Li, Shihao 1,2,3; Yu, Yang 1,2,3; Xiang, Jianhai 2,3; Li, Fuhua 1,2,3,4
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2020-10-01
卷号	111 页码:11
关键词	Antimicrobial peptide Crustin Intestinal microbiota Homeostasis Crustacean
ISSN号	0145-305X
DOI	10.1016/j.dci.2020.103756
英文摘要	Crustins are a family of antimicrobial peptides (AMP) with multiple functions, including antimicrobial activity, capability of protease inhibition, phagocytosis promotion, and wound healing in crustaceans. Till present, several members of crustins have been identified and their activities were studied. However, there are still less investigations on how they play functions in vivo. Here, we identified a novel crustin with an atypical WAP domain, LvCrustin I-1, which is mainly distributed in tissues, including intestine, gill, epidermis and stomach of the shrimp Litopenaeus vannamei. The expression level of LvCrustin I-1 was significantly up-regulated at 3 h, 6 h, 12 h, and 24 h after Vibrio parahaemolyticus infection. Knockdown of LvCrustin I-1 with dsRNA resulted in a significant increase of the bacteria number in hepatopancreas of shrimp upon V. parahaemolyticus infection, showing that LvCrustin I-1 participated in pathogen infection process. Recombinant LvCrustin I-1 protein showed microorganism-binding activity rather than antibacterial activity against tested bacteria. Furthermore, significant difference existed between the intestinal microbiota in shrimp before and after LvCrustin I-1 knockdown based on the result of alpha and NMDS analyses. Knockdown of LvCrustin I-1 increased the proportion of Demequina, Nautella, Propionibacterium, Anaerospora and decreased the proportion of Bacteroidia and Vibrio. These data suggest that LvCrustin I-1 might perform its immunological function through modulation of the intestinal microbiota homeostasis rather than direct inhibition of bacterial growth in shrimp.
资助项目	National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[41776158] ; China Agricultural Research System[CARS-48]
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000551873700004
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/167993]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lv, Xinjia,Li, Shihao,Yu, Yang,et al. The immune function of a novel crustin with an atypical WAP domain in regulating intestinal microbiota homeostasis in Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,111:11.
APA	Lv, Xinjia,Li, Shihao,Yu, Yang,Xiang, Jianhai,&Li, Fuhua.(2020).The immune function of a novel crustin with an atypical WAP domain in regulating intestinal microbiota homeostasis in Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,111,11.
MLA	Lv, Xinjia,et al."The immune function of a novel crustin with an atypical WAP domain in regulating intestinal microbiota homeostasis in Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 111(2020):11.