Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production

doi:10.3390/md18050229

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	Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production
	Gao, Baoyan 1; Huang, Luodong 1; Lei, Xueqing 1; Meng, Ge 1; Liu, Jianguo 2,3; Zhang, Chengwu 1
刊名	MARINE DRUGS
	2020-05-01
卷号	18 期号:5 页码:17
关键词	Lobosphaera bisecta light intensity medium replacement lipid arachidonic acid transcriptome analysis
DOI	10.3390/md18050229
通讯作者	Zhang, Chengwu(tzhangcw@jnu.edu.cn)
英文摘要	Arachidonic acid (AA) is an omega-6 long-chain polyunsaturated fatty acid and is important for human health. The coccoid green microalga Lobosphaera bisecta has been reported to be able to accumulate high AA content under certain conditions. Nutrient management and light intensity had significant effects on the biomass and accumulation of lipids and AA in L. bisecta SAG2043. Both a high nitrogen concentration (18 mM) and high light intensity (bilateral light-300 mu mol m(-2) s(-1)) were beneficial to the growth of L. bisecta, and the replacement of culture medium further enhanced the biomass, which reached 8.9 g L-1. Low nitrogen concentration (3.6 mM) and high light significantly promoted the accumulation of lipids and AA. The highest lipid and AA content reached 54.0% and 10.8% of dry weight, respectively. Lipid compositions analysis showed that 88.2% of AA was distributed within the neutral lipids. We then reconstructed the lipid metabolic pathways of L. bisecta for the first time, and demonstrated that the upregulation of a key desaturase and elongase in the Delta 6 pathway was conducive to the accumulation of fatty acids toward AA synthesis. L. bisecta SAG2043 exhibits high biomass, lipid and AA production. It may be a potential candidate for AA production.
资助项目	Sinopec joint program of China Petroleum and Chemical Corporation[ST18005-2] ; Guangzhou science and technology innovation program[201907010005] ; Fundamental Research Funds for the Central Universities[21619305] ; Blue Life of Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[MS2019N001]
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MDPI
WOS记录号	WOS:000541031900007
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167777]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, Chengwu
作者单位	1.Jinan Univ, Res Ctr Hydrobiol, Dept Ecol, Guangzhou 510632, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Gao, Baoyan,Huang, Luodong,Lei, Xueqing,et al. Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production[J]. MARINE DRUGS,2020,18(5):17.
APA	Gao, Baoyan,Huang, Luodong,Lei, Xueqing,Meng, Ge,Liu, Jianguo,&Zhang, Chengwu.(2020).Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production.MARINE DRUGS,18(5),17.
MLA	Gao, Baoyan,et al."Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production".MARINE DRUGS 18.5(2020):17.