The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles

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	The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles
	Wang, Y.2,3; Wang, B. J.2; Liu, M.2; Jiang, K. Y.2; Wang, M. Q.4,5; Wang, L.1,2,6
刊名	ISJ-INVERTEBRATE SURVIVAL JOURNAL
	2020
卷号	17 页码:108-116
关键词	Cherax quadricarinatus C-type lectin innate immunity
ISSN号	1824-307X
通讯作者	Wang, M. Q.(wangmengqiang@ouc.edu.cn) ; Wang, L.(leiwang@qdio.ac.cn)
英文摘要	As pattern recognition receptors (PRRs), C-type lectins (CTLs) have important roles in the recognition and clearance of pathogens by the innate immune system. In the present study, the first Cherax quadricarinatus CTL gene (designated CqCTL) was cloned and characterized. The complete cDNA sequence of CqCTL contained an open reading frame (ORF) of 543 bp, which encoded a protein of 180 amino acids. A carbohydrate recognition domain (CRD) containing four conserved cysteines (Cys(48), Cys(59), Cys(76), Cys(177)) and the EPD (Glu(80)-Pro(81)-Asn(82)) and QPD (Gln(146)-Pro(147)-Asn(148)) motifs were identified in the deduced amino acid sequence of CqCTL. The deduced tertiary structure of CqCTL revealed two a helices,five beta sheets and two disulfide bonds. CqCTL exhibited high similarity with previously identified CTLs from other species. The mRNA transcripts of CqCTL were ubiquitously detectable in all the tested tissues, with the highest expression level in hepatopancreas. These results provide useful information on the potential role of CqCTL in the innate immune system of C. quadricarinatus, and lay the foundation for further studies on the CTLs of crustacean.
资助项目	National Key R&D Program of China[2019YFD0900401] ; STS Program of Chinese Academy of Sciences, Fujian Provincial Department of Science and Technology[2019T3035]
WOS研究方向	Immunology ; Zoology
语种	英语
出版者	INVERTEBRATE SURVIVAL JOURNAL
WOS记录号	WOS:000536611100001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167575]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, M. Q.; Wang, L.
作者单位	1.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ocean Univ China, MOE Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China 5.Natl Lab Marine Sci & Technol, Ctr Marine Mol Biotechnol, Qingdao 266237, Peoples R China 6.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Peoples R China
推荐引用方式 GB/T 7714	Wang, Y.,Wang, B. J.,Liu, M.,et al. The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2020,17:108-116.
APA	Wang, Y.,Wang, B. J.,Liu, M.,Jiang, K. Y.,Wang, M. Q.,&Wang, L..(2020).The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles.ISJ-INVERTEBRATE SURVIVAL JOURNAL,17,108-116.
MLA	Wang, Y.,et al."The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles".ISJ-INVERTEBRATE SURVIVAL JOURNAL 17(2020):108-116.