Transcriptome analysis reveals the regulation of the shrimp STAT on host chitin-binding domain containing proteins and energy metabolism process during WSSV infection

doi:10.1016/j.fsi.2020.03.026

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Transcriptome analysis reveals the regulation of the shrimp STAT on host chitin-binding domain containing proteins and energy metabolism process during WSSV infection
	Yang, Feifei 1,2,3,5; Li, Shihao 1,3,5; Xiang, Jianhai 1,5; Zhao, Xingming 4; Li, Fuhua 1,3,5
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-05-01
卷号	100 页码:345-357
关键词	STAT WSSV Chitin binding Glycolysis Lipid metabolism Litopenaeus vannamei
ISSN号	1050-4648
DOI	10.1016/j.fsi.2020.03.026
通讯作者	Li, Shihao(lishihao@qdio.ac.cn)
英文摘要	JAK/STAT signaling pathway is suggested to enhance the infection of WSSV in crustaceans. However, the regulation mechanism of this process is not quite clear. Here, comparative transcriptomic analysis was performed among shrimps before and after Litopenaeus vannamei STAT (LvSTAT) was silenced by dsRNA approach during WSSV infection. Differentially expressed genes (DEGs) common in the STAT-interfered groups and control groups at different times after WSSV infection were analyzed to acquire the genes probably regulated by LvSTAT. DEGs annotation and further GO terms enrichment analyses revealed that the identified DEGs mainly contained two categories, chitin-binding domain containing proteins and energy metabolism related genes. The former mainly included cuticle proteins, thrombospondins (TSPs) and peritrophin, while the later mainly included hexose catabolic process and glycolysis related genes. Two cuticle proteins and two TSPs were further studied to learn their expression changes during WSSV infection. They were significantly regulated during WSSV infection, implying the involvement of chitin-binding domain containing protein in the invasion process of WSSV. Systematic analysis on the glycolysis and lipid synthesis pathway demonstrated that silencing of LvSTAT could reduce the glycolysis efficiency and the production of lipids. It could be speculated that a favorable function of LvSTAT for WSSV replication existed by regulating the energy metabolism of the host. Through revealing the main category of genes and biological processes regulated by STAT, our study could shed new light on the roles of JAK/STAT signaling pathway in shrimp during virus infection.
资助项目	China Agriculture Research System-48[CARS-48] ; China Postdoctoral Science Foundation[2017M612361] ; General Program of National Natural Science Foundation of China[31772880] ; General Program of National Natural Science Foundation of China[41776158]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000523603600037
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167026]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Shihao
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Lianyungang 222005, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Fudan Univ, Inst Sci & Technol Brain Inspired Intelligence, Shanghai 200433, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Yang, Feifei,Li, Shihao,Xiang, Jianhai,et al. Transcriptome analysis reveals the regulation of the shrimp STAT on host chitin-binding domain containing proteins and energy metabolism process during WSSV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2020,100:345-357.
APA	Yang, Feifei,Li, Shihao,Xiang, Jianhai,Zhao, Xingming,&Li, Fuhua.(2020).Transcriptome analysis reveals the regulation of the shrimp STAT on host chitin-binding domain containing proteins and energy metabolism process during WSSV infection.FISH & SHELLFISH IMMUNOLOGY,100,345-357.
MLA	Yang, Feifei,et al."Transcriptome analysis reveals the regulation of the shrimp STAT on host chitin-binding domain containing proteins and energy metabolism process during WSSV infection".FISH & SHELLFISH IMMUNOLOGY 100(2020):345-357.