Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846)

doi:10.4194/1303-2712-v20_6_02

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	Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846)
	Tan, Xungang 2,3; Sui, Yulei 1,2,3; Li, Meijie 1,2,3; Jiao, Shuang 2,3; Wu, Zhihao 2,3; You, Feng 2,3
刊名	TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
	2020-06-01
卷号	20 期号:6 页码:421-429
关键词	Abdomen Gonads Phylogenetic analysis Teleost fish
ISSN号	1303-2712
DOI	10.4194/1303-2712-v20_6_02
英文摘要	The nanos gene family plays a critical role during germline development in a wide array of organisms. The characteristics of the gene structure and function of nanos1, a nanos homolog, vary from species to species. Herein, we isolated the full-length cDNA of a nanos1 homolog in the olive flounder, Paralichthys olivaceus, and analyzed its expression pattern. The full-length cDNA sequence of the olive flounder nanos1 has 1215 base pairs (bp) and contains a 112 bp 5'-untranslated region, a 684 bp open reading frame that encodes a 228 AA peptide, and a 419 bp 3'- untranslated region. Phylogenetic analyses showed that the Nanos1 homolog of the olive flounder grouped with the nanos1 of teleost fish. We detected nanos1 mRNA in all stages of embryogenesis using RT-PCR analyses. Our whole mount in situ hybridization results showed that nands1 was expressed in the diencephalon, midbrain, hindbrain, nose, medulla oblongata, retina, abdomen, and somatic gonadal cells. Our data indicate that the expression pattern of nanos1 is not consistent from species to species, supporting that nanos1 had different functions in different organisms.
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	CENTRAL FISHERIES RESEARCH INST
WOS记录号	WOS:000506048700002
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/164424]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Tan, Xungang
作者单位	1.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Tan, Xungang,Sui, Yulei,Li, Meijie,et al. Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846)[J]. TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES,2020,20(6):421-429.
APA	Tan, Xungang,Sui, Yulei,Li, Meijie,Jiao, Shuang,Wu, Zhihao,&You, Feng.(2020).Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846).TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES,20(6),421-429.
MLA	Tan, Xungang,et al."Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846)".TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES 20.6(2020):421-429.