Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions

doi:10.1111/are.14427

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions
	Gong, Fengying 1,2; Zhang, Chunhui 1,2,3; Zhang, Litao 1,3,4; Liu, Jianguo 1,3,4
刊名	AQUACULTURE RESEARCH
	2019-12-04
页码	9
关键词	astaxanthin carotenoids geometrical isomerization haematococcus pluvialis prenyltransferases
ISSN号	1355-557X
DOI	10.1111/are.14427
英文摘要	In this study, carotenoid contents of Haematococcus pluvialis during outdoor high light cultivation were measured, moreover, changes of astaxanthin geometrical isomers and biotic factors which may affect isomerization were investigated. During the incubation, contents of both astaxanthin and its precursors (zeaxanthin and canthaxanthin) increased over time, whereas the relative content of lutein decreased. Contents of all astaxanthin isomers increased, while the proportion of different astaxanthin geometrical isomers fluctuated during the incubation. All-trans-astaxanthin of total astaxanthin (T/A) was higher during the astaxanthin accumulation phase than that in the cell transformation phase, which was in contrast to results of 13-cis-astaxanthin of total astaxanthin (13C/A) and 9-cis-astaxanthin of total astaxanthin (9C/A). Farnesyl diphosphate synthase (trans (2E,6E)-FPPS, EC2.5.1.10) and geranylgeranyl diphosphate synthase (GGPS, EC2.5.1.29), the key proteins involved in geometrical isomerization, decreased during the astaxanthin accumulation phase. Moreover, the presence of cis (2Z,6E)-FPPS was firstly confirmed in H. pluvialis by HPLC-MS/MS shotgun method. The results indicated the biotic factor (trans-FPPS, cis-FPPS and GGPS) may play an important role in astaxanthin geometrical isomerization of H. pluvialis, but not a crucial role. This study would help in optimizing the regulation of astaxanthin geometrical isomers in H. pluvialis, with great significance in theory and production.
资助项目	National Natural Science Foundation of China[U1706209] ; National Natural Science Foundation of China[31572639]
WOS研究方向	Fisheries
语种	英语
出版者	WILEY
WOS记录号	WOS:000500304300001
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/163789]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 4.Yunnan Alphy Biotech Co Ltd, Natl Local Joint Engn Res Ctr Haematococcus Pluvi, Chuxiong, Peoples R China
推荐引用方式 GB/T 7714	Gong, Fengying,Zhang, Chunhui,Zhang, Litao,et al. Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions[J]. AQUACULTURE RESEARCH,2019:9.
APA	Gong, Fengying,Zhang, Chunhui,Zhang, Litao,&Liu, Jianguo.(2019).Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions.AQUACULTURE RESEARCH,9.
MLA	Gong, Fengying,et al."Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions".AQUACULTURE RESEARCH (2019):9.