Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives

doi:10.3389/fbioe.2020.00833

CORC > 海洋研究所 > 中国科学院海洋研究所

	Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives
	Sun, Shenmei 1,2; Ding, Yamei 3; Liu, Min 1; Xian, Mo 1; Zhao, Guang 1,4
刊名	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
	2020-07-23
卷号	8 页码:13
关键词	carbon source glucose ethanol acetate acetyl-CoA bioproduction
ISSN号	2296-4185
DOI	10.3389/fbioe.2020.00833
通讯作者	Zhao, Guang(zhaoguang@sdu.edu.cn)
英文摘要	Poly(3-hydroxybutyrate) (PHB) is a biodegradable and biocompatible thermoplastic, and synthesized from the central metabolite acetyl-CoA. The acetyl-CoA synthesis from glucose presents low atomic economy due to the release of CO(2)in pyruvate decarboxylation. As ethanol and acetate can be converted into acetyl-CoA directly, they were used as carbon source for PHB production in this study. The reductase mutant AdhE A267T/E568K was introduced intoEscherichia colito enable growth on ethanol, and acetate utilization was improved by overexpression of acetyl-CoA synthetase ACS. Comparison of the PHB production using glucose, ethanol or acetate as sole carbon source showed that the production and yield from ethanol was much higher than those from glucose and acetate, and metabolome analysis revealed the differences in metabolism of glucose, ethanol and acetate. Furthermore, other acetyl-CoA derived chemicals including 3-hydroxypropionate and phloroglucinol were produced from those three feedstocks, and similar results were achieved, suggesting that ethanol could be a suitable carbon source for the production of acetyl-CoA derivatives.
资助项目	NSFC[31722001] ; NSFC[31670089] ; Natural Science Foundation of Shandong Province[JQ201707] ; National Defense Science and Technology Innovation Zone Foundation of China ; Defense Industrial Technology Development Program[JCKY2018130B005] ; Shandong University
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000560293700001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168186]
专题	中国科学院海洋研究所
通讯作者	Zhao, Guang
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Shandong Univ, State Key Lab Microbial Technol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Sun, Shenmei,Ding, Yamei,Liu, Min,et al. Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2020,8:13.
APA	Sun, Shenmei,Ding, Yamei,Liu, Min,Xian, Mo,&Zhao, Guang.(2020).Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,13.
MLA	Sun, Shenmei,et al."Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2020):13.