Spatial high resolution of actin filament organization by PeakForce atomic force microscopy

doi:10.1111/cpr.12670

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Spatial high resolution of actin filament organization by PeakForce atomic force microscopy
	Liu, L ; Wei, YH ; Liu, JY ; Wang, KZ ; Zhang, JJ ; Zhang, P ; Zhou, Y ; Li, B
刊名	CELL PROLIFERATION
	2019
卷号	53 期号:1 页码:-
关键词	ION CONDUCTANCE MICROSCOPY CYTOSKELETON MICROVILLI DYNAMICS STIFFNESS CELLS
ISSN号	0960-7722
DOI	10.1111/cpr.12670
文献子类	期刊论文
英文摘要	Objectives To investigate the heterogeneous feature of actin filaments (ACFs) associated with the cellular membrane in HeLa and HCT-116 cells at the nanoscale level. Materials and Methods Fluorescence microscopy coupled with atomic force microscopy (AFM) was used to identify and characterize ACFs of cells. The distribution of ACFs was detected by Fluor-488-phalloidin-labelled actin. The morphology of the ACFs was probed by AFM images. The spatial correlation of the microvilli and ACFs was explored with different forces of AFM loading on cells. Results Intricate but ordered structures of the actin cytoskeletons associated with cellular membrane were characterized and revealed. Two different layers of ACFs with distinct structural organizations were directly observed in HCT-116 and HeLa cells. Bundle-shaped ACFs protruding the cellular membrane forming the microvilli, and the network ACFs underneath the cellular membrane were resolved with high resolution under near-physiological conditions. Approximately 14 nm lateral resolution was achieved when imaging single ACF beneath the cellular membrane. On the basis of the observed spatial distribution of the ultrastructure of the ACF organization, a model for this organization of ACFs was proposed. Conclusions We revealed the two layers of the ACF organization in Hela and HCT-116 cells. The resolved heterogeneous structures at the nanoscale level provide a spatial view of the ACFs, which would contribute to the understanding of the essential biological functions of the actin cytoskeleton.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32212]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China; 2.Chengdu Univ Tradit Chinese Med, Sch Basic Med, Chengdu 611137, Sichuan, Peoples R China 3.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Div Phys Biol & Bioimagin, Shanghai 201800, Peoples R China; 4.Fourth Mil Med Univ, Xian, Shaanxi, Peoples R China;
推荐引用方式 GB/T 7714	Liu, L,Wei, YH,Liu, JY,et al. Spatial high resolution of actin filament organization by PeakForce atomic force microscopy[J]. CELL PROLIFERATION,2019,53(1):-.
APA	Liu, L.,Wei, YH.,Liu, JY.,Wang, KZ.,Zhang, JJ.,...&Li, B.(2019).Spatial high resolution of actin filament organization by PeakForce atomic force microscopy.CELL PROLIFERATION,53(1),-.
MLA	Liu, L,et al."Spatial high resolution of actin filament organization by PeakForce atomic force microscopy".CELL PROLIFERATION 53.1(2019):-.