Solidifying framework nucleic acids with silica

doi:10.1038/s41596-019-0184-0

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Solidifying framework nucleic acids with silica
	Jing, XX ; Zhang, F ; Pan, MC ; Dai, XP ; Li, J ; Wang, LH ; Liu, XG ; Yan, H ; Fan, CH
刊名	NATURE PROTOCOLS
	2019
卷号	14 期号:8 页码:2416-2436
关键词	DNA ORIGAMI FOLDING DNA NANOSTRUCTURES NANOPARTICLES ASSEMBLIES DELIVERY GROWTH
ISSN号	1754-2189
DOI	10.1038/s41596-019-0184-0
文献子类	期刊论文
英文摘要	Soft matter can serve as a template to guide the growth of inorganic components with well-controlled structural features. However, the limited design space of conventional organic and biomolecular templates restricts the complexity and accuracy of templated growth. In past decades, the blossoming of structural DNA nanotechnology has provided us with a large reservoir of delicate-framework nucleic acids with design precision down to a single base. Here, we describe a DNA origami silicification (DOS) approach for generating complex silica composite nanomaterials. By utilizing modified silica sol-gel chemistry, pre-hydrolyzed silica precursor clusters can be uniformly coated onto the surface of DNA frameworks; thus, user-defined DNA-silica hybrid materials with similar to 3-nm precision can be achieved. More importantly, this method is applicable to various 1D, 2D and 3D DNA frameworks that range from 10 to > 1,000 nm. Compared to pure DNA scaffolds, a tenfold increase in the Young's modulus (E modulus) of these composites was observed, owing to their soft inner core and solid silica shell. We further demonstrate the use of solidified DNA frameworks to create 3D metal plasmonic devices. This protocol provides a platform for synthesizing inorganic materials with unprecedented complexity and tailored structural properties. The whole protocol takes similar to 10 d to complete.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32209]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med, Shanghai, Peoples R China; 2.Univ Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Div Phys Biol, Chinese Acad Sci,Shanghai Synchrotron Radiat Faci, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Bioimaging Ctr, Chinese Acad Sci,Shanghai Synchrotron Radiat Faci, Shanghai, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Sch Chem & Chem Engn, Shanghai, Peoples R China; 5.Biodesign Inst, Ctr Mol Design & Biomimet, Tempe, AZ USA; 6.Arizona State Univ, Sch Mol Sci, Tempe, AZ USA; 7.East China Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Jing, XX,Zhang, F,Pan, MC,et al. Solidifying framework nucleic acids with silica[J]. NATURE PROTOCOLS,2019,14(8):2416-2436.
APA	Jing, XX.,Zhang, F.,Pan, MC.,Dai, XP.,Li, J.,...&Fan, CH.(2019).Solidifying framework nucleic acids with silica.NATURE PROTOCOLS,14(8),2416-2436.
MLA	Jing, XX,et al."Solidifying framework nucleic acids with silica".NATURE PROTOCOLS 14.8(2019):2416-2436.