Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance

doi:10.1021/acs.jafc.9b04589

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 资源植物与生物技术所级重点实验室

	Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance
	Yu, Lujun 3; Chen, Qnfang 3; Peng, Yujun 3; Xie, Lijuan 3; Liu, Di 3; Han, Muqian 2,3; Chen, Feng 1; Xiao, Shi 3; Huang, Junchao 4; Li, Juan 2,3
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2019-11-13
卷号	67 期号:45 页码:12590-12598
关键词	carotenoid astaxanthin oxidation ABA BKT pathogen
ISSN号	0021-8561
DOI	10.1021/acs.jafc.9b04589
通讯作者	Huang, Junchao(huangjc@mail.kib.ac.cn) ; Li, Juan(adalee619@163.com)
英文摘要	Carotenoids play key roles in photosynthesis and photoprotection. Few multicellular plants produce the ketocarotenoid astaxanthin, a strong antioxidant; however, Arabidopsis thaliana lines overexpressing the Chlamydomonas reinhardtii beta-carotene ketolase (CrBKT) accumulated high amounts of astaxanthin in the leaves. In this study, we investigated the changed regulation of key metabolic pathways and the tolerance of the engineered plants to biotic and abiotic stresses resulting from the heterologous expression of CrBKT. Transcriptome analysis identified 1633 and 1722 genes that were differentially expressed in the leaves and siliques, respectively, of CrBKT-overexpressing plants (line CR5) as compared to wild-type Arabidopsis. These genes were enriched in the carotenoid biosynthetic pathways, and plant hormone biosynthesis and signaling pathways. In particular, metabolic profiling showed that, as compared to the wild-type leaves and siliques, overexpression of CrBKT increased the levels of most amino acids, but decreased the contents of sugars and carbohydrates. Furthermore, CR5 plants had lower sensitivity to abscisic acid (ABA) and increased tolerance to oxidative stress. CRS plants also exhibited enhanced resistance to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Our study provides insight into the regulation of carotenoids and the related pathways, which may be involved in plant response to oxidative stress and pathogen infection.
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
WOS记录号	WOS:000497263900029
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/70437]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
通讯作者	Huang, Junchao; Li, Juan
作者单位	1.Shenzhen Univ, Inst Adv Study, Shenzhen 518000, Guangdong, Peoples R China 2.Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China 3.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol & Guangdong Prov Key Lab, Guangzhou 510275, Guangdong, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yu, Lujun,Chen, Qnfang,Peng, Yujun,et al. Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(45):12590-12598.
APA	Yu, Lujun.,Chen, Qnfang.,Peng, Yujun.,Xie, Lijuan.,Liu, Di.,...&Li, Juan.(2019).Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(45),12590-12598.
MLA	Yu, Lujun,et al."Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.45(2019):12590-12598.